在氧气受限条件下，以麦芽糖作为碳源时酿酒酵母中的阶段特异性基因表达。
Phase-specific gene expression in Saccharomyces cerevisiae, using maltose as carbon source under oxygen-limiting conditions.
作者信息
Donalies U E, Stahl U
机构信息
Technische Universität Berlin, Institut für Biotechnologie, Fachgebiet Mikrobiologie und Genetik, Gustav-Meyer-Allee 25, 13355 Berlin, Germany.
出版信息
Curr Genet. 2001 May;39(3):150-5. doi: 10.1007/s002940100195.
The transcription of ten stress-response genes was investigated under oxygen-limiting conditions with maltose and glucose, respectively. Six of these genes (HSP12, HSP26, HSP30, HSP78, HSP82 and HSP104) showed expression only during the stationary phase. HSP12 and HSP104 were transcribed 10 h earlier with maltose than with glucose. Fermentation in wort yielded similar results to the maltose-based medium. HSP12, HSP26 and HSP30 were highly expressed. Thus, the HSP26 and the HSP30 promoter can be used for late, phase-specific expression of the desired genes with glucose or maltose as carbon source, and HSP12 with glucose only. MET14, a gene important for sulfite formation, was overexpressed in wort, using the HSP26 promoter during the stationary phase.
分别在以麦芽糖和葡萄糖为碳源的限氧条件下，研究了十个应激反应基因的转录情况。其中六个基因（HSP12、HSP26、HSP30、HSP78、HSP82和HSP104）仅在稳定期表达。HSP12和HSP104在以麦芽糖为碳源时比以葡萄糖为碳源时提前10小时转录。麦芽汁发酵产生的结果与以麦芽糖为基础的培养基相似。HSP12、HSP26和HSP30高表达。因此，HSP26和HSP30启动子可用于以葡萄糖或麦芽糖为碳源时所需基因的后期、阶段特异性表达，而HSP12仅用于以葡萄糖为碳源时。MET14是对亚硫酸盐形成很重要的一个基因，在稳定期使用HSP26启动子在麦芽汁中过表达。
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