Régnacq M, Boucherie H
Laboratoire de Génétique, URA CNRS 542, Talence, France.
Curr Genet. 1993 May-Jun;23(5-6):435-42. doi: 10.1007/BF00312631.
Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.
通过差异杂交，分离出了一个在进入稳定期时优先表达的基因。后续分析表明，该基因对应于一个热休克基因。已确定其核苷酸序列。结果显示它编码一个332个氨基酸的蛋白质。未发现与任何已知蛋白质有相似之处。该蛋白质具有很强的疏水性，预计定位于膜上。与此假设相符的是，对稳定期细胞的膜蛋白分析表明，在多拷贝载体上携带该基因的菌株会过量产生一种30 kDa的蛋白质。该蛋白质可被针对基因产物N端部分的抗体识别。鉴于其在热休克反应中的诱导作用及其产物的表观分子量，该基因被命名为HSP30。
