Namiduru E S, Ozdemir Y, Kutlar I, Ersoy U
Department of Biochemistry and Clinical Biochemistry, University of Gaziantep, Faculty of Medicine, Sehitkamil, Gaziantep, Turkey.
Arch Gynecol Obstet. 2001 May;265(2):73-5. doi: 10.1007/s004040000142.
The prolidase activity in serum and in erythrocytes was measured in 28 healthy mothers and in the cord blood of their newborn babies in using a modified Chinard method. 45 healthy non-pregnant women aged between 15-36 years formed a control group. Biochemical parameters (CK, BUN, C-peptid, AFP, Uric acid) were also measured. The serum and erythrocyte prolidase activities in maternal blood were 45.8+/-13.4 U/L and 37.8+/-2.7 U/g Hb respectively. There was no significant difference in the enzyme activities between pregnant women and the control group (p>0.05). However serum and erythrocyte prolidase activity in cord blood (20.3+/-8.2 U/L and 31.6+/-7.3 U/g Hb) was significantly different when compared with control group (53.4+/-14.7 U/L in serum and 42.3+/-10.3 U/g Hb in erythrocyte, p<0.001). There was a significant correlation between maternal and cord blood serum enzyme activity (r: 0.76 p<0.01). This correlation was also shown in erythrocyte prolidase activities of both groups (r: 0.49, p<0.05). Cord blood prolidase activity was positively correlated with birth weight (r: 0.89, p<0.01). Prolidase activity in cord blood was low even though collagen turnover is increased in fetal growth.
采用改良的奇纳德方法，对28名健康母亲及其新生儿的脐带血中的血清和红细胞中的脯氨酰二肽酶活性进行了测定。45名年龄在15至36岁之间的健康非孕妇组成了对照组。还测量了生化参数（肌酸激酶、尿素氮、C肽、甲胎蛋白、尿酸）。母亲血液中的血清和红细胞脯氨酰二肽酶活性分别为45.8±13.4 U/L和37.8±2.7 U/g血红蛋白。孕妇与对照组之间的酶活性无显著差异（p>0.05）。然而，与对照组相比，脐带血中的血清和红细胞脯氨酰二肽酶活性（分别为20.3±8.2 U/L和31.6±7.3 U/g血红蛋白）有显著差异（血清中为53.4±14.7 U/L，红细胞中为42.3±10.3 U/g血红蛋白，p<0.001）。母亲和脐带血血清酶活性之间存在显著相关性（r：0.76，p<0.01）。两组的红细胞脯氨酰二肽酶活性也显示出这种相关性（r：0.49，p<0.05）。脐带血脯氨酰二肽酶活性与出生体重呈正相关（r：0.89，p<0.01）。尽管胎儿生长过程中胶原蛋白更新增加，但脐带血中的脯氨酰二肽酶活性仍然较低。
