Lamartiniere C A, Dieringer C S, Kita E, Lucier G W
Biochem J. 1979 May 15;180(2):313-8. doi: 10.1042/bj1800313.
The hepatic microsomal enzyme UDP-glucuronyltransferase undergoes a complex developmental pattern in which enzyme activity is first detectable on the 18th day of gestation in rats. Prepubertal activities are similar for males and females. However, postpubertal sexual differentiation of enzyme activity occurs in which male activities are twice those of females. Neonatal administration of testosterone propionate or diethylstilboestrol to intact animals resulted in lowered UDP-glucuronyltransferase activity in liver microsomal fractions of adult male rats, whereas no changes were observed in the adult females and prepubertal male and female animals. Neonatal administration of testosterone propionate and diethylstilboestrol adversely affected male reproductive-tract development as evidenced by decreased weights of testes, seminal vesicles and ventral prostate. Diethylstilboestrol also markedly decreased spermatogenesis. Hypophysectomy of adult male rats resulted in negative modulation of microsomal UDP-glucuronyltransferase and prevented the sexual differentiation of enzyme activity. In contrast hypophysectomy had no effect on female UDP-glucuronyltransferase activity. A pituitary transplant under the kidney capsule was not capable of reversing the enzyme effects of hypophysectomy, therefore suggesting that the male pituitary factor(s) responsible for positive modulation of UDP-glucuronyltransferase might be under hypothalamic control in the form of a releasing factor. Neonatal testosterone propionate and diethylstilboestrol administration apparently interfered with the normal sequence of postpubertal UDP-glucuronyltransferase sexual differentiation.
肝脏微粒体酶UDP - 葡萄糖醛酸基转移酶呈现出复杂的发育模式，在大鼠妊娠第18天首次可检测到酶活性。青春期前雄性和雌性的酶活性相似。然而，青春期后酶活性出现性别分化，雄性活性是雌性的两倍。对完整动物在新生期给予丙酸睾酮或己烯雌酚，导致成年雄性大鼠肝脏微粒体部分的UDP - 葡萄糖醛酸基转移酶活性降低，而成年雌性以及青春期前的雄性和雌性动物未观察到变化。新生期给予丙酸睾酮和己烯雌酚对雄性生殖道发育产生不利影响，表现为睾丸、精囊和腹侧前列腺重量减轻。己烯雌酚还显著降低精子发生。成年雄性大鼠垂体切除导致微粒体UDP - 葡萄糖醛酸基转移酶的负调节，并阻止了酶活性的性别分化。相比之下，垂体切除对雌性UDP - 葡萄糖醛酸基转移酶活性没有影响。将垂体移植到肾被膜下不能逆转垂体切除对酶的影响，因此表明负责UDP - 葡萄糖醛酸基转移酶正调节的雄性垂体因子可能以释放因子的形式受下丘脑控制。新生期给予丙酸睾酮和己烯雌酚显然干扰了青春期后UDP - 葡萄糖醛酸基转移酶性别分化的正常顺序。
