Sueiro R A, Araujo M, Suárez S, Garrido M J
Laboratory of Microbiology, Institute of Food Analysis and Research (IIAA), University of Santiago, 15706 Santiago de Compostela, Spain.
Mutagenesis. 2001 Jul;16(4):303-7. doi: 10.1093/mutage/16.4.303.
The mutagenicity of bisphenol A diglycidyl ether (BADGE), its first and second hydrolysis products (the diol epoxide and bis-diol of BADGE, respectively) and the bis-chlorohydrin of BADGE were investigated using the Ames Salmonella assay with strains TA98, TA100, TA1535 and TA1537. The assays were performed in the absence and presence of various concentrations of rat liver S9 fraction. The results obtained confirm the mutagenic power of BADGE in strains TA100 and TA1535 and show a positive response to the diol epoxide of BADGE in these strains, although the latter compound was approximately 10 times less potent than the former. A lack of mutagenic activity of the bis-diol of BADGE and the chlorohydrin under study is also shown. These findings suggest that BADGE and, to a much lesser extent, the diol epoxide of BADGE may constitute a genotoxic hazard, but not the bis-diol or bis-chlorohydrin of BADGE.
采用鼠伤寒沙门氏菌TA98、TA100、TA1535和TA1537菌株的Ames试验，研究了双酚A二缩水甘油醚（BADGE）、其一级和二级水解产物（分别为BADGE的二醇环氧化物和双二醇）以及BADGE的双氯醇的诱变性。试验在有无不同浓度大鼠肝脏S9组分的情况下进行。所得结果证实了BADGE在TA100和TA1535菌株中的诱变能力，并表明这些菌株对BADGE的二醇环氧化物有阳性反应，尽管后一种化合物的效力比前一种低约10倍。研究还表明，BADGE的双二醇和氯醇没有诱变活性。这些发现表明，BADGE以及程度小得多的BADGE二醇环氧化物可能构成遗传毒性危害，但BADGE的双二醇或双氯醇则不会。
