双酚A二缩水甘油醚的水解产物及氯羟基衍生物雌激素活性的体外测定
In vitro assay of hydrolysis and chlorohydroxy derivatives of bisphenol A diglycidyl ether for estrogenic activity.
作者信息
Nakazawa H, Yamaguchi A, Inoue K, Yamazaki T, Kato K, Yoshimura Y, Makino T
机构信息
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2-4-41, Shinagawa-ku, Tokyo 142-8501, Japan.
出版信息
Food Chem Toxicol. 2002 Dec;40(12):1827-32. doi: 10.1016/s0278-6915(02)00165-5.
Bisphenol A diglycidyl ether (BADGE) is an epoxy resin monomer. Epoxy-based solution coatings are used in many applications as additives for a variety of plastic coatings in food packaging. It is well known that unreacted BADGE can migrate from epoxy-based packing materials into foods. Not only BADGE but also its derivatives can easily migrate into foods and it is likely that we intake BADGE and its derivatives through food or drink. Recently, endocrine disrupting chemicals (EDCs) have attracted attention because they have been shown to affect reproduction in wildlife. The estrogenic activity of BADGE derivatives has not previously been investigated. Therefore, we investigated the estrogenic activity of the BADGE derivatives, dihydrolysed BADGE (BADGE-4OH) and chlorohydroxy BADGE (BADGE-2Cl), using breast cancer cell (T47D) proliferation assay and estrogen receptor (ER) (alpha) binding assay. These chemicals exhibited T47D cell proliferation at concentrations of 10(-14)-10(-4) M. However, these chemicals did not bind to ER (alpha) in the binding assay.
双酚A二缩水甘油醚（BADGE）是一种环氧树脂单体。环氧基溶液涂料在许多应用中用作食品包装中各种塑料涂料的添加剂。众所周知，未反应的BADGE会从环氧基包装材料迁移到食品中。不仅BADGE，其衍生物也很容易迁移到食品中，我们很可能通过食物或饮料摄入BADGE及其衍生物。最近，内分泌干扰化学物质（EDCs）引起了关注，因为它们已被证明会影响野生动物的繁殖。BADGE衍生物的雌激素活性此前尚未得到研究。因此，我们使用乳腺癌细胞（T47D）增殖试验和雌激素受体（ER）（α）结合试验，研究了BADGE衍生物二水解BADGE（BADGE-4OH）和氯羟基BADGE（BADGE-2Cl）的雌激素活性。这些化学物质在浓度为10（-14）-10（-4）M时表现出T47D细胞增殖。然而，在结合试验中，这些化学物质未与ER（α）结合。
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