Drezek R, Brookner C, Pavlova I, Boiko I, Malpica A, Lotan R, Follen M, Richards-Kortum R
Biomedical Engineering Program and Department of Electrical and Computer Engineering, University of Texas at Austin, Austin, TX, USA.
Photochem Photobiol. 2001 Jun;73(6):636-41. doi: 10.1562/0031-8655(2001)073<0636:AMOFCT>2.0.CO;2.
Fluorescence spectroscopy offers an effective, noninvasive approach to the detection of precancers in multiple organ sites. Clinical studies have demonstrated that fluorescence spectroscopy can provide highly sensitive, specific and cost-effective diagnosis of cervical precancers. However, the underlying biochemical mechanisms responsible for differences in the fluorescence spectra of normal and dysplastic tissue are not fully understood. We designed a study to assess the differences in autofluorescence of normal and dysplastic cervical tissue. Transverse, fresh tissue sections were prepared from colposcopically normal and abnormal biopsies in a 34-patient study. Autofluorescence images were acquired at 380 and 460 nm excitation. Results showed statistically significant increases in epithelial fluorescence intensity (arbitrary units) at 380 nm excitation in dysplastic tissue (106 +/- 39) relative to normal tissue (85 +/- 30). The fluorophore responsible for this increase is possibly reduced nicotinamide adenine dinucleotide. Stromal fluorescence intensities in the dysplastic samples decreased at both 380 nm (102 +/- 34 [dysplasia] vs 151 +/- 44 [normal]) and 460 nm excitation (93 +/- 35 [dysplasia] vs 137 +/- 49 [normal]), wavelengths at which collagen is excited. Decreased redox ratio (17-40% reduction) in dysplastic tissue sections, indicative of increased metabolic activity, was observed in one-third of the paired samples. These results provide valuable insight into the biological basis of the differences in fluorescence of normal and precancerous cervical tissue.
荧光光谱法为检测多个器官部位的癌前病变提供了一种有效、非侵入性的方法。临床研究表明，荧光光谱法能够对宫颈的癌前病变进行高度灵敏、特异且经济高效的诊断。然而，正常组织与发育异常组织荧光光谱差异背后的生化机制尚未完全明晰。我们设计了一项研究来评估正常与发育异常宫颈组织的自体荧光差异。在一项针对34名患者的研究中，从阴道镜检查正常和异常活检样本制备横向新鲜组织切片。在380纳米和460纳米激发波长下采集自体荧光图像。结果显示，相对于正常组织（85±30），发育异常组织在380纳米激发波长下上皮荧光强度（任意单位）有统计学意义的增加（106±39）。导致这种增加的荧光团可能是还原型烟酰胺腺嘌呤二核苷酸。发育异常样本的基质荧光强度在380纳米（发育异常组为102±34，正常组为151±44）和460纳米激发波长（发育异常组为93±35，正常组为137±49）下均降低，这两个波长是激发胶原蛋白的波长。在三分之一的配对样本中，观察到发育异常组织切片的氧化还原比降低（降低17 - 40%），这表明代谢活性增加。这些结果为正常和癌前宫颈组织荧光差异的生物学基础提供了有价值的见解。
