Okamoto M, Sato T, Shirai H, Imai Y
Department of Removable Prosthodontics, Okayama University Dental School, Okayama City, Okayama, Japan.
J Oral Rehabil. 2001 Jun;28(6):553-9. doi: 10.1046/j.1365-2842.2001.00694.x.
The purpose of this study was to investigate bone dynamics in denture supporting tissue under continuous pressure in the diabetic condition by using bone histomorphometry in relation to initial intensity of continuous pressure exerted through the denture base. The experimental denture base, which was designed to load initial continuous pressure of 0.0, 1.0, 10.0 or 20.0 kPa to the denture supporting tissue, was applied to the molar region of hard palate of streptozotocin-induced diabetic rats. Fluorescent labelled palatal bone tissue was stained with Villanueva bone stain and was prepared for the undecalcified grinding section. In 0.0 kPa group, no bone resorption was observed and bone formation was transiently inhibited after the denture insertion. In 1.0 kPa group, although no bone resorption was observed, the beginning of bone formation after the inhibition of bone formation was later than that in 0.0 kPa group and bone formation dynamics after the resumption of bone formation was similar to that in 0.0 kPa group. In 10.0 and 20.0 kPa groups, bone resorption was observed until 3 and 4 weeks, respectively, and the amount of bone resorption for each group was 60 +/- 16 and 87 +/- 18 microm, respectively. The resumption of bone formation in 10.0 and 20.0 kPa groups were observed at the same stage with 1.0 kPa group, and the bone formation dynamics after the resumption of bone formation in 10.0 and 20.0 kPa groups were also similar to that in 0.0 kPa group. From the results of this study, it was revealed that bone formation following bone resorption did not cause equivalent recovery of the bone surface level to the level observed in the case without bone resorption in the diabetic condition.
本研究的目的是通过骨组织形态计量学，研究在糖尿病状态下，义齿基托持续施加压力时，义齿支持组织中的骨动力学，该压力与通过义齿基托施加的初始持续压力强度相关。将设计用于向义齿支持组织施加0.0、1.0、10.0或20.0 kPa初始持续压力的实验性义齿基托，应用于链脲佐菌素诱导的糖尿病大鼠硬腭的磨牙区。用Villanueva骨染色对荧光标记的腭骨组织进行染色，并制备不脱钙磨片。在0.0 kPa组，未观察到骨吸收，义齿插入后骨形成短暂受到抑制。在1.0 kPa组，虽然未观察到骨吸收，但骨形成受抑制后骨形成的开始时间比0.0 kPa组晚，骨形成恢复后的骨形成动力学与0.0 kPa组相似。在10.0和20.0 kPa组，分别在3周和4周时观察到骨吸收，每组的骨吸收量分别为60±16和87±18微米。10.0和20.0 kPa组骨形成的恢复与1.0 kPa组在同一阶段观察到，10.0和20.0 kPa组骨形成恢复后的骨形成动力学也与0.0 kPa组相似。从本研究结果可知，在糖尿病状态下，骨吸收后的骨形成并未使骨表面水平恢复到未发生骨吸收时观察到的水平。
