Nasr-Esfahani M H, Razavi S, Mardani M
Isfahan Fertility and Infertility Center, Isfahan, Iran.
J Assist Reprod Genet. 2001 Apr;18(4):219-25. doi: 10.1023/a:1009412130417.
To consider the relationship between different sperm nuclear maturity tests and in vitro fertilization (IVF) rate, in order to select the most sensitive, specific, and independent factor(s) for prediction of in vitro fertilization.
Infertile couples (101) were randomly selected from IVF candidates referred to Isfahan Fertility and Infertility center. Semen samples were collected on the day of oocyte recovery. Following routine semen analysis, major portion of the semen was prepared for routine IVF insemination and the remaining was used for following sperm nuclear maturity tests: chromomycin A3 (CMA3), aniline blue, sodium dodecyl sulphate (SDS) test, and acridine orange test with or without heat shock (87 degrees C, 5 min). Sperms (200) were evaluated for each test. The results were recorded and analyzed for their correlation to fertilization rate, using correlation coefficient, logistic regression analysis, student t-test, and receiver operating characteristics (ROC) curve.
Among these tests, aniline blue and CMA3, and semen parameters, sperm morphology, and sperm motility showed a significant correlation with fertilization rate. Using logistic regression analysis, sperm morphology and CMA3 were the only independent factors related to in vitro fertilization. ROC curves showed that among above tests, CMA3 is the most specific and sensitive for sperm nuclear maturity.
Among CMA3, aniline blue, SDS test, and acridine orange, CMA3 was the most sensitive and specific test that can be used along with routine semen analysis for more precise prediction of fertilization rate.
探讨不同精子核成熟度检测方法与体外受精（IVF）率之间的关系，以筛选出预测体外受精最敏感、特异且独立的因素。
从转诊至伊斯法罕生育与不孕中心的IVF候选不孕夫妇中随机选取101对。在取卵日采集精液样本。进行常规精液分析后，将大部分精液用于常规IVF授精，其余用于以下精子核成熟度检测：放线菌素A3（CMA3）、苯胺蓝、十二烷基硫酸钠（SDS）试验以及有无热休克（87℃，5分钟）的吖啶橙试验。每项检测评估200个精子。记录结果并分析其与受精率的相关性，采用相关系数、逻辑回归分析、学生t检验和受试者工作特征（ROC）曲线。
在这些检测中，苯胺蓝、CMA3以及精液参数、精子形态和精子活力与受精率显著相关。通过逻辑回归分析，精子形态和CMA3是与体外受精相关的仅有的独立因素。ROC曲线显示，在上述检测中，CMA3对精子核成熟度最特异且敏感。
在CMA3、苯胺蓝、SDS试验和吖啶橙中，CMA3是最敏感且特异的检测方法，可与常规精液分析一起用于更精确地预测受精率。
