Iranpour F G, Nasr-Esfahani M H, Valojerdi M R, al-Taraihi T M
Department of Anatomy, Medical School, Tarbiat Modarres University, Tehran, Iran.
J Assist Reprod Genet. 2000 Jan;17(1):60-6. doi: 10.1023/a:1009406231811.
Our purpose was to investigate the association between percentage chromomycin A3 (CMA3) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate.
Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides. CMA3 positivity is expressed as the percentage in 200 spermatozoa.
Percentage of CMA3 positivity showed not only a negative correlation with fertilization rate but also a significant difference between fertilizing and nonfertilizing patients. Moreover, percentage of CMA3-positive spermatozoa showed a negative correlation with count and percentage motility and a positive correlation with percentage of abnormal morphology. Percentage of CMA3 positivity also had a positive correlation with some abnormalities of head such as amorphous and macrocephaly. Ultrastructural study showed chromatin unpackaging in high CMA3-positive semen samples in comparison with low CMA3-positive semen samples.
There is a close relationship among fertilization rate, sperm parameters, and CMA3 positivity and CMA3 could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.
我们的目的是研究精子的放线菌素A3（CMA3）阳性率与一些精子参数及体外受精率之间的关联。
从139名男性中收集精子，用磷酸盐缓冲液（PBS）洗涤，固定于甲醇/冰醋酸（3:1）中，然后涂片于载玻片上。CMA3阳性率以200个精子中的百分比表示。
CMA3阳性率不仅与受精率呈负相关，而且在受精和未受精患者之间存在显著差异。此外，CMA3阳性精子的百分比与精子计数和活动率百分比呈负相关，与异常形态百分比呈正相关。CMA3阳性率还与一些头部异常如无定形和巨头畸形呈正相关。超微结构研究显示，与低CMA3阳性精液样本相比，高CMA3阳性精液样本中染色质解包装。
受精率、精子参数和CMA3阳性率之间存在密切关系，CMA3可被视为不孕症治疗前评估男性生育能力的有用工具。
