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本文引用的文献

1
Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence?卵胞浆内单精子注射后多余胚胎的囊胚发育：父系影响？
Hum Reprod. 1998 Jun;13(6):1632-7. doi: 10.1093/humrep/13.6.1632.
2
Chromatin packaging and morphology in ejaculated human spermatozoa: evidence of hidden anomalies in normal spermatozoa.射出的人类精子中的染色质包装与形态：正常精子中隐藏异常的证据
Mol Hum Reprod. 1996 Mar;2(3):139-44. doi: 10.1093/molehr/2.3.139.
3
Chromomycin A3-staining as an indicator of protamine deficiency and fertilization.以放线菌素A3染色作为鱼精蛋白缺乏和受精的指标。
Int J Androl. 1996 Feb;19(1):23-7. doi: 10.1111/j.1365-2605.1996.tb00429.x.
4
Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection.精子染色质异常会影响胞浆内单精子注射后的解聚。
Hum Reprod. 1996 Apr;11(4):837-43. doi: 10.1093/oxfordjournals.humrep.a019263.
5
Mammalian sperm DNA susceptibility to in situ denaturation associated with the presence of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay.通过末端脱氧核苷酸转移酶测定法测量，哺乳动物精子DNA对与DNA链断裂相关的原位变性的敏感性。
J Androl. 1995 Jan-Feb;16(1):80-7.
6
Relation of mammalian sperm chromatin heterogeneity to fertility.哺乳动物精子染色质异质性与生育能力的关系。
Science. 1980 Dec 5;210(4474):1131-3. doi: 10.1126/science.7444440.
7
A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence.一种通过吖啶橙（AO）荧光对男性生育能力进行实际评估的测试。
Fertil Steril. 1984 Jul;42(1):87-91. doi: 10.1016/s0015-0282(16)47963-x.
8
The influence of seminal characteristics on the success rate of human in vitro fertilization.
Fertil Steril. 1984 Sep;42(3):400-5. doi: 10.1016/s0015-0282(16)48080-5.
9
Corrective measures and pregnancy outcome in in vitro fertilization in patients with severe sperm morphology abnormalities.
Fertil Steril. 1988 Aug;50(2):283-7. doi: 10.1016/s0015-0282(16)60074-2.
10
The use of in vitro fertilization to evaluate putative tests of human sperm function.使用体外受精来评估人类精子功能的假定检测方法。
Fertil Steril. 1988 Feb;49(2):272-7. doi: 10.1016/s0015-0282(16)59715-5.

放线菌素A3染色作为评估男性生育能力的一种有用工具。

Chromomycin A3 staining as a useful tool for evaluation of male fertility.

作者信息

Iranpour F G, Nasr-Esfahani M H, Valojerdi M R, al-Taraihi T M

机构信息

Department of Anatomy, Medical School, Tarbiat Modarres University, Tehran, Iran.

出版信息

J Assist Reprod Genet. 2000 Jan;17(1):60-6. doi: 10.1023/a:1009406231811.

DOI:10.1023/a:1009406231811
PMID:10754785
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3455193/
Abstract

PURPOSE

Our purpose was to investigate the association between percentage chromomycin A3 (CMA3) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate.

METHODS

Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides. CMA3 positivity is expressed as the percentage in 200 spermatozoa.

RESULTS

Percentage of CMA3 positivity showed not only a negative correlation with fertilization rate but also a significant difference between fertilizing and nonfertilizing patients. Moreover, percentage of CMA3-positive spermatozoa showed a negative correlation with count and percentage motility and a positive correlation with percentage of abnormal morphology. Percentage of CMA3 positivity also had a positive correlation with some abnormalities of head such as amorphous and macrocephaly. Ultrastructural study showed chromatin unpackaging in high CMA3-positive semen samples in comparison with low CMA3-positive semen samples.

CONCLUSION

There is a close relationship among fertilization rate, sperm parameters, and CMA3 positivity and CMA3 could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.

摘要

目的

我们的目的是研究精子的放线菌素A3（CMA3）阳性率与一些精子参数及体外受精率之间的关联。

方法

从139名男性中收集精子，用磷酸盐缓冲液（PBS）洗涤，固定于甲醇/冰醋酸（3:1）中，然后涂片于载玻片上。CMA3阳性率以200个精子中的百分比表示。

结果

CMA3阳性率不仅与受精率呈负相关，而且在受精和未受精患者之间存在显著差异。此外，CMA3阳性精子的百分比与精子计数和活动率百分比呈负相关，与异常形态百分比呈正相关。CMA3阳性率还与一些头部异常如无定形和巨头畸形呈正相关。超微结构研究显示，与低CMA3阳性精液样本相比，高CMA3阳性精液样本中染色质解包装。

结论

受精率、精子参数和CMA3阳性率之间存在密切关系，CMA3可被视为不孕症治疗前评估男性生育能力的有用工具。