Moellenbeck D J, Peters M L, Bing J W, Rouse J R, Higgins L S, Sims L, Nevshemal T, Marshall L, Ellis R T, Bystrak P G, Lang B A, Stewart J L, Kouba K, Sondag V, Gustafson V, Nour K, Xu D, Swenson J, Zhang J, Czapla T, Schwab G, Jayne S, Stockhoff B A, Narva K, Schnepf H E, Stelman S J, Poutre C, Koziel M, Duck N
Pioneer Hi-Bred International, Inc., 7250 NW 62nd Avenue, P.O. Box 552, Johnston, IA 50131-0552, USA.
Nat Biotechnol. 2001 Jul;19(7):668-72. doi: 10.1038/90282.
Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kDa and 44 kDa. Corn rootworm larvae fed on corn roots expressing the proteins showed histopathological symptoms in the midgut epithelium.
对共表达两种来自苏云金芽孢杆菌（Bt）的新型δ-内毒素的玉米进行的田间试验表明，其对西部玉米根虫（Diabrotica virgifera virgifera LeConte）造成的根部损害具有防护作用。防护水平超过了化学杀虫剂所提供的防护水平。在这种细菌中，这些蛋白质在生长周期的孢子形成阶段形成晶体，由一个操纵子编码，分子量分别为14 kDa和44 kDa。取食表达这些蛋白质的玉米根的玉米根虫幼虫，其肠道上皮出现了组织病理学症状。
