Arts M J, Haenen G R, Voss H P, Bast A
Department of Pharmacology and Toxicology, Faculty of Medicine, Maastricht University, PO Box 616, 6200 MD, Maastricht, The Netherlands.
Food Chem Toxicol. 2001 Aug;39(8):787-91. doi: 10.1016/s0278-6915(01)00020-5.
The effect of antioxidants is often executed in complex biological mixtures where various interactions may take place. Therefore, the antioxidant capacity of antioxidants in blood plasma is examined. The assay used is the trolox equivalent antioxidant capacity (TEAC). This method gives the antioxidant capacity of a compound by measuring spectrophotometrically the disappearance of the blue/green stable ABTS [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] radical, caused by scavenging. The results show that the antioxidant capacity of quercetin, rutin, catechin or 7-monohydroxyethylrutoside (monoHER) and blood plasma is not additive. This is partly due to interactions between the antioxidant and plasma proteins. However, the antioxidant capacity of alpha-tocopherol, which also binds to protein, is not affected by the interaction. This means that besides the antioxidant capacity of the compound itself, the environment in which the antioxidant has to execute his function is important.
抗氧化剂的作用通常在可能发生各种相互作用的复杂生物混合物中发挥。因此，要检测血浆中抗氧化剂的抗氧化能力。所使用的检测方法是特罗克斯当量抗氧化能力（TEAC）。该方法通过分光光度法测量由清除作用导致的蓝色/绿色稳定ABTS[2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）]自由基的消失，从而得出化合物的抗氧化能力。结果表明，槲皮素、芦丁、儿茶素或7-单羟乙基芦丁（单HER）与血浆的抗氧化能力并非相加关系。这部分是由于抗氧化剂与血浆蛋白之间的相互作用。然而，同样与蛋白质结合的α-生育酚的抗氧化能力不受这种相互作用的影响。这意味着，除了化合物本身的抗氧化能力外，抗氧化剂发挥作用的环境也很重要。
