Kong H H, Hwang M Y, Kim H K, Chung D I
Department of Parasitology, Kyungpook National University School of Medicine, Taeyu 700-422, Korea.
Korean J Parasitol. 2001 Jun;39(2):151-60. doi: 10.3347/kjp.2001.39.2.151.
Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes encoding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.
从棘阿米巴属赫利种cDNA文库中随机挑选435个克隆进行测序，共产生了387个表达序列标签（EST）。基于BLAST搜索结果，130个克隆（34.4%）被鉴定为编码表面蛋白、DNA酶、能量产生或其他代谢相关酶、激酶和磷酸酶、蛋白酶、信号转导蛋白、结构和细胞骨架蛋白、细胞周期相关蛋白、转录因子、转录和翻译机制以及转运蛋白的基因。大多数基因（88.5%）是在棘阿米巴属中首次鉴定的。虽然有15个克隆与公共数据库中棘阿米巴属的基因匹配，但其中12个克隆是肌动蛋白基因，它是本研究中表达最频繁的基因。棘阿米巴属的这些EST将为把该生物体作为细胞骨架或细胞运动、信号转导、转录和翻译调控等生物学研究的模型系统进行研究提供有价值的信息。这些结果也将为阐明棘阿米巴属在人类肉芽肿性阿米巴脑炎或角膜炎发病机制中的因素提供线索。
