el-Sayed N M, Alarcon C M, Beck J C, Sheffield V C, Donelson J E
Department of Biochemistry, University of Iowa, Iowa City 52242, USA.
Mol Biochem Parasitol. 1995 Jul;73(1-2):75-90. doi: 10.1016/0166-6851(95)00098-l.
A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.
从罗德西亚布氏锥虫血流克隆的cDNA文库和剪接前导子亚文库中随机挑选克隆，共产生了518个表达序列标签（EST）。根据与公共数据库中113个独特基因的匹配情况，鉴定出205个（39%）克隆。其中，71个cDNA与不相关生物体中编码代谢酶、信号转导蛋白、转录因子、核糖体蛋白、组蛋白、增殖相关蛋白和硫醇寡肽酶等的基因有显著相似性。313个cDNA与数据库中的任何其他序列均无关联。这些cDNA EST为探索新的锥虫特异性基因和该寄生虫的基因组组织提供了新的研究途径，同时也为鉴定锥虫与其他生物体中表达基因的同源物提供了资源。
