Azadniv Mitra, Doida Yukio, Miller Morton W., Brayman Andrew A., Meltzer Richard S.
Department of Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14624.
Echocardiography. 1996 Jan;13(1):45-56. doi: 10.1111/j.1540-8175.1996.tb00866.x.
To test the hypothesis that the full extent of in vitro cell lysis due to ultrasound becomes evident with time lapse following insonation, human erythrocytes (2% hematocrit) in autologous plasma were mixed with Albunex(R), a pulse echo contrast agent, and exposed to 1-MHz, continuous-wave ultrasound (US) (5 W/cm(2) SPTA intensity) for 60 seconds while in a rotating (200 rpm) dialysis membrane vessel. Exposed and sham-exposed samples were subsequently assayed for hemolysis colorimetrically, either immediately or after a delay of 3 hours. Hemolysis was dependent on the interval between US exposure and assay, with significantly greater lysis evident with delayed assay. There was also temporality in lytic yield with sample number, i.e., with time postpreparation of the blood sample, US-induced cell lysis decreased. The temporality of lytic yield was eliminated by maintenance at ice water temperatures, or by waiting about 1 hour before beginning treatments. The collective data indicate that the full extent of US-induced cell lysis is not evident upon assay immediately after insonation, and that with time postpreparation and preinsonation, erythrocytes may undergo changes in sensitivity to US. (ECHOCARDIOGRAPHY, Volume 13, January 1996)
为了验证超声所致体外细胞溶解的全部程度在超声照射后的时间推移过程中变得明显这一假设，将自体血浆中含2%血细胞比容的人红细胞与脉冲回声造影剂Albunex(R)混合，并在旋转（200转/分钟）的透析膜容器中，暴露于1兆赫的连续波超声（US）（5瓦/平方厘米的空间峰值时间平均强度）下60秒。随后，对暴露组和假暴露组样本立即或延迟3小时后进行比色法溶血测定。溶血程度取决于超声暴露与测定之间的间隔时间，延迟测定时溶血明显更严重。随着样本数量增加，溶血产量也存在时间相关性，即血液样本制备后的时间越长，超声诱导的细胞溶解越少。通过在冰水温度下保存或在开始处理前等待约1小时，可消除溶血产量的时间相关性。总体数据表明，超声照射后立即进行测定时，超声诱导的细胞溶解的全部程度并不明显，并且在样本制备和超声照射前的时间里，红细胞对超声的敏感性可能会发生变化。（《超声心动图学》，第13卷，1996年1月）
