Emmerick Leandro Santiago, Schwarz Marcos Gustavo Araujo, Corrêa Paloma Rezende, Piñero Sindy Licette, Gomes Leonardo Henrique Ferreira, Almeida Ana Maria Mazotto, Valente Richard Hemmi, Degrave Wim Maurits Sylvain, Mendonça-Lima Leila
Laboratório de Genômica Aplicada e Bioinovações, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Laboratório de Biologia Molecular Aplicada à Micobactérias, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
Front Microbiol. 2025 Apr 25;16:1558006. doi: 10.3389/fmicb.2025.1558006. eCollection 2025.
The Mycobacterium genus remains highly relevant today due to the rising incidence of tuberculosis and opportunistic infections caused by environmental mycobacteria. While much is known about , and , studies focusing on environmental mycobacteria remain limited. These microorganisms are globally distributed and have been identified in diverse biomes, including the Atlantic Forest. This study aims to provide a characterization of four mycobacterial strains isolated from the Atlantic Forest, assessing their metabolic capabilities and biotechnological potential. We investigated the presence of cellulases and proteases and conducted an initial profiling of secreted proteins. Furthermore, the examination of shared antigens and infection kinetics within macrophages offered insights into the ecological and pathogenic potential of these isolates. From a public health perspective, antigenic similarities between these environmental microorganisms and the BCG vaccine strain may influence the efficacy of BCG in protecting against diseases such as tuberculosis. Continued research on these and other environmental isolates, particularly within Brazil's highly biodiverse ecosystems, holds promise for advancing scientific knowledge and contributing to human health.
由于结核病发病率上升以及环境分枝杆菌引起的机会性感染，分枝杆菌属如今仍然具有高度相关性。虽然人们对[此处原文缺失相关内容]了解很多，但针对环境分枝杆菌的研究仍然有限。这些微生物分布于全球，在包括大西洋森林在内的不同生物群落中都有发现。本研究旨在对从大西洋森林分离出的四株分枝杆菌菌株进行特征描述，评估它们的代谢能力和生物技术潜力。我们研究了纤维素酶和蛋白酶的存在情况，并对分泌蛋白进行了初步分析。此外，对巨噬细胞内共同抗原和感染动力学的研究为这些分离株的生态和致病潜力提供了见解。从公共卫生角度来看，这些环境微生物与卡介苗菌株之间的抗原相似性可能会影响卡介苗预防结核病等疾病的功效。对这些以及其他环境分离株的持续研究，特别是在巴西生物多样性极高的生态系统中进行的研究，有望推动科学知识的进步并为人类健康做出贡献。
