Hattery D, Chernomordik V, Loew M, Gannot I, Gandjbakhche A
Laboratory of Integrative and Medical Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Opt Soc Am A Opt Image Sci Vis. 2001 Jul;18(7):1523-30. doi: 10.1364/josaa.18.001523.
An analytical solution is developed to quantify a site-specific fluorophore lifetime perturbation that occurs, for example, when the local metabolic status is different from that of surrounding tissue. This solution may be used when fluorophores are distributed throughout a highly turbid media and the site of interest is embedded many mean scattering distances from the source and the detector. The perturbation in lifetime is differentiated from photon transit delays by random walk theory. This analytical solution requires a priori knowledge of the tissue-scattering and absorption properties at the excitation and emission wavelengths that may be obtained from concurrent time-resolved reflection measurements. Additionally, the solution has been compared with the exact, numerically solved solution. Thus the presented solution forms the basis for practical lifetime imaging in turbid media such as tissue.
开发了一种解析解，用于量化特定位置的荧光团寿命扰动，例如，当局部代谢状态与周围组织不同时所发生的扰动。当荧光团分布在高度浑浊的介质中，且感兴趣的位置距离源和探测器有许多平均散射距离时，该解析解可用。寿命的扰动通过随机游走理论与光子传输延迟区分开来。该解析解需要激发和发射波长处组织散射和吸收特性的先验知识，这些知识可从同步时间分辨反射测量中获得。此外，该解已与精确的数值解进行了比较。因此，所提出的解构成了在诸如组织等浑浊介质中进行实际寿命成像的基础。
