Chernomordik Victor, Gandjbakhche Amir H, Hassan Moinuddin, Pajevic Sinisa, Weiss George H
Section on Analytical and Functional Biophotonics, Program on Pediatric Imaging and Tissue Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892.
Opt Commun. 2010 Dec 1;283(23):4832-4839. doi: 10.1016/j.optcom.2010.06.099.
We develop an analytic model of time-resolved fluorescent imaging of photons migrating through a semi-infinite turbid medium bounded by an infinite plane in the presence of a single stationary point fluorophore embedded in the medium. In contrast to earlier models of fluorescent imaging in which photon motion is assumed to be some form of continuous diffusion process, the present analysis is based on a continuous-time random walk (CTRW) on a simple cubic lattice, the object being to estimate the position and lifetime of the fluorophore. Such information can provide information related to local variations in pH and temperature with potential medical significance. Aspects of the theory were tested using time-resolved measurements of the fluorescence from small inclusions inside tissue-like phantoms. The experimental results were found to be in good agreement with theoretical predictions provided that the fluorophore was not located too close to the planar boundary, a common problem in many diffusive systems.
我们建立了一个解析模型，用于在存在嵌入介质中的单个静止点荧光团的情况下，对穿过由无限平面界定的半无限浑浊介质迁移的光子进行时间分辨荧光成像。与早期的荧光成像模型不同，早期模型假定光子运动是某种形式的连续扩散过程，而本分析基于简单立方晶格上的连续时间随机游走（CTRW），目的是估计荧光团的位置和寿命。此类信息可提供与pH值和温度的局部变化相关的信息，具有潜在的医学意义。使用对组织样体模内小内含物的荧光进行时间分辨测量来测试该理论的各个方面。结果发现，只要荧光团离平面边界不太近（这是许多扩散系统中的常见问题），实验结果就与理论预测高度吻合。
