Wozniak A, Drewa G, Chesy G, Rakowski A, Rozwodowska M, Olszewska D
Departments of Biology and Physiology, Ludwik Rydygier Medical University, Military Sports Centre, PMR, Bydgoszcz, Poland.
Med Sci Sports Exerc. 2001 Jul;33(7):1109-13. doi: 10.1097/00005768-200107000-00007.
The aim of this work was an evaluation of the influence of physical exercise in high-altitude conditions (about 2000 m above sea level) on thiobarbituric acid reactive substances (TBARS) and superoxide dismutase (SOD) and catalase (CAT) activities in 10 kayakers and 10 rowers.
During their training, the sportsmen performed different kinds of static and dynamic efforts. The blood samples were taken from the cubital vein on the control day at low altitude and at high altitude, and on the 4th, 10th, and 18th days of the training camp before and after exercise. The TBARS and lactic acid concentrations in blood plasma and SOD and CAT activities in erythrocytes were measured.
A statistically significant increase of SOD and CAT activities in erythrocytes after exercise on the 4th, 10th, and 18th days of training was found. The TBARS concentration in erythrocytes decreased in a statistically significant way after the end of the 10th day of exercise (P < 0.01), and on 18th day it more than doubled (P < 0.001) when preexercise values were compared with postexercise values of each day. A statistically significant increase of TBARS concentration in blood plasma was observed only after the end of exercise on the 10th day-using the same comparison as above. A statistically significant increased lactic acid concentration in blood plasma was noticed both on 4th or 18th days of training.
The results obtained show the increasing generation of oxygen-derived free radicals and the compensatory intensification of SOD and CAT activities after training in altitude (high mountain) conditions.
本研究旨在评估在高海拔条件下（海拔约2000米）进行体育锻炼对10名皮划艇运动员和10名赛艇运动员体内硫代巴比妥酸反应性物质（TBARS）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的影响。
在训练期间，运动员进行了不同类型的静态和动态运动。在低海拔和高海拔的对照日，以及训练营第4天、第10天和第18天运动前后，从肘静脉采集血样。检测血浆中TBARS和乳酸浓度以及红细胞中SOD和CAT活性。
发现在训练第4天、第10天和第18天运动后，红细胞中SOD和CAT活性有统计学意义的增加。运动第10天结束后，红细胞中TBARS浓度显著下降（P<0.01），与每天运动前值相比，第18天其增加了一倍多（P<0.001）。仅在第10天运动结束后，血浆中TBARS浓度有统计学意义的增加（采用上述相同比较方法）。在训练第4天和第18天，血浆中乳酸浓度均有统计学意义的增加。
所得结果表明，在高海拔（高山）条件下训练后，氧衍生自由基的生成增加，SOD和CAT活性代偿性增强。
