Nie Li-Hua, Zhao Hui-Chun, Wang Xu, Yi Lin, Lu Yan, Jin Lin-Pei, Ma Hui-Min
Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Anal Bioanal Chem. 2002 Dec;374(7-8):1187-90. doi: 10.1007/s00216-002-1553-y. Epub 2002 Oct 31.
A simple, rapid and sensitive chemiluminescence (CL) method was proposed for the determination of lomefloxacin (LFX). This method is based on the fact that the weak CL from the redox reaction of Ce(4+)-Na(2)SO(3) can be greatly enhanced by the complex of Tb(3+)-LFX. The CL intensity is directly proportional to the concentration of LFX in the range 2.0x10(-9) to 1.0x10(-5) mol L(-1), and the detection limit (S/N=3) is 1.1x10(-9) mol L(-1). This method has been applied to the detection of LFX in pharmaceutical preparation, urine and serum samples. Recoveries were in the range 95-105%. The CL mechanism of Ce(4+)-Na(2)SO(3)-Tb(3+)-LFX system was proposed to be an intermolecular energy transfer from excited SO(2)(*) to LFX and an intramolecular energy transfer from LFX to Tb(3+).
提出了一种简单、快速且灵敏的化学发光（CL）方法用于测定洛美沙星（LFX）。该方法基于以下事实：Ce(4+)-Na₂SO₃氧化还原反应产生的微弱化学发光可被Tb(3+)-LFX络合物极大增强。化学发光强度在2.0×10⁻⁹至1.0×10⁻⁵ mol L⁻¹范围内与LFX浓度成正比，检测限（S/N = 3）为1.1×10⁻⁹ mol L⁻¹。该方法已应用于药物制剂、尿液和血清样品中LFX的检测。回收率在95%至105%之间。Ce(4+)-Na₂SO₃-Tb(3+)-LFX体系的化学发光机理被认为是从激发态SO₂(*)到LFX的分子间能量转移以及从LFX到Tb(3+)的分子内能量转移。
