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本文引用的文献

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Structural and kinetic characterization of an acyl transferase ribozyme.一种酰基转移酶核酶的结构与动力学特性
J Am Chem Soc. 1998 Feb 18;120(6):1151-6. doi: 10.1021/ja972472s.
2
Guilt by association: the arginine case revisited.牵连有罪：再论精氨酸案例
RNA. 2000 Apr;6(4):499-510. doi: 10.1017/s1355838200000145.
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The scene of a frozen accident.一起冻伤事故的现场。
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RNA-ligand chemistry: a testable source for the genetic code.RNA-配体化学：遗传密码的一个可验证来源。
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Ribozyme-catalyzed tRNA aminoacylation.核酶催化的tRNA氨基酰化作用。
Nat Struct Biol. 2000 Jan;7(1):28-33. doi: 10.1038/71225.
6
Specific, rapid synthesis of Phe-RNA by RNA.通过RNA特异性、快速合成苯丙氨酸RNA。
Proc Natl Acad Sci U S A. 1999 May 11;96(10):5470-5. doi: 10.1073/pnas.96.10.5470.
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Nucleotide analog interference mapping.核苷酸类似物干扰图谱分析
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8
A map of the binding site for catalytic domain 5 in the core of a group II intron ribozyme.II组内含子核酶核心中催化结构域5的结合位点图谱。
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9
Defining functional groups, core structural features and inter-domain tertiary contacts essential for group II intron self-splicing: a NAIM analysis.定义II组内含子自我剪接所必需的功能基团、核心结构特征和结构域间三级接触：一项NAIM分析
EMBO J. 1998 Dec 1;17(23):7091-104. doi: 10.1093/emboj/17.23.7091.
10
Genetic code origins: tRNAs older than their synthetases?遗传密码的起源：转运RNA比其合成酶更古老？
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一种小螺旋-环RNA作为转氨酰化催化剂。

A minihelix-loop RNA acts as a trans-aminoacylation catalyst.

作者信息

Lee N, Suga H

机构信息

Department of Chemistry, State University of New York at Buffalo, 14260-3000, USA.

出版信息

RNA. 2001 Jul;7(7):1043-51. doi: 10.1017/s1355838201010457.

DOI:10.1017/s1355838201010457
PMID:11453065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1370145/
Abstract

We previously reported a bifunctional ribozyme that catalyzes self-aminoacylation and subsequent acyl-transfer to a tRNA. The ribozyme selectively recognizes a biotinyl-glutamine substrate, and charges the tRNA molecule in trans. Structurally, there are two catalytic domains, referred to as glutamine-recognition (QR) and acyl-transferase (ATRib). We report here the essential catalytic core of the QR domain as determined by extensive biochemical probing, mutation, and structural minimization. The minimal core of the QR domain is a 29-nt helix-loop RNA, which is also able to glutaminylate ATRib in trans. Its amino acid binding site is embedded in an 11-nt cluster that is adjacent to the loop that interacts with the ATRib domain. Our study shows that a minihelix-loop RNA can act as a trans-aminoacylation catalyst, which lends support for the critical role of minihelix-loops in the early evolution of the aminoacylation system.

摘要

我们之前报道过一种双功能核酶，它能催化自身氨酰化以及随后的酰基转移至tRNA。该核酶选择性识别生物素化谷氨酰胺底物，并在反式作用下使tRNA分子负载氨基酸。在结构上，有两个催化结构域，分别称为谷氨酰胺识别（QR）和酰基转移酶（ATRib）。我们在此报告通过广泛的生化探测、突变和结构最小化确定的QR结构域的关键催化核心。QR结构域的最小核心是一个29个核苷酸的螺旋-环RNA，它也能够在反式作用下使ATRib谷氨酰胺化。其氨基酸结合位点嵌入在一个11个核苷酸的簇中，该簇与与ATRib结构域相互作用的环相邻。我们的研究表明，一个小螺旋-环RNA可以作为反式氨酰化催化剂，这为小螺旋-环在氨酰化系统早期进化中的关键作用提供了支持。