用于确认淋病奈瑟菌的玻片凝集技术与Minitek系统的比较。
Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae.
作者信息
Hampton K D, Stallings R A, Wasilauskas B L
出版信息
J Clin Microbiol. 1979 Sep;10(3):290-2. doi: 10.1128/jcm.10.3.290-292.1979.
Abstract
The Phadebact gonococcus test was performed on 314 bacterial isolates to compare the coagglutination technique with the Minitek system for the confirmation of Neisseria gonorrhoeae. Direct testing, using colonies of N. gonorrhoeae mixed with the Phadebact gonococcus test reagents, produced noninterpretable results in many cases. However, an alternative method, described by the manufacturer, of heating several colonies in distilled water for 20 min before testing alleviated most interpretation difficulties. Complete agreement was achieved with both the Phadebact and Minitek tests for all 236 isolates of N. gonorrhoeae. Of the 78 nongonococcal isolates tested with Phadebact, two isolates gave false-positive results, whereas one isolate was reported as noninterpretable. The Phadebact gonococcus test appears to be a simple and rapid alternative to confirmatory biochemical procedures for N. gonorrhoeae.
摘要
对314株细菌分离株进行了Phadebact淋球菌试验，以比较凝集技术和Minitek系统用于确认淋病奈瑟菌的情况。直接检测时，将淋病奈瑟菌菌落与Phadebact淋球菌试验试剂混合，在许多情况下产生了无法解读的结果。然而，制造商描述的一种替代方法，即在检测前将几个菌落置于蒸馏水中加热20分钟，缓解了大多数解读困难。对所有236株淋病奈瑟菌分离株，Phadebact试验和Minitek试验结果完全一致。在用Phadebact检测的78株非淋球菌分离株中，有两株出现假阳性结果，而有一株报告为无法解读。Phadebact淋球菌试验似乎是一种简单快速的方法，可替代用于淋病奈瑟菌的确证性生化程序。
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