在改良纽约市培养基上培养后通过协同凝集进行淋球菌鉴定的快速性和可靠性。
Rapidity and reliability of gonococcal identification by coagglutination after culture on modified New York City medium.
作者信息
Young H, McMillan A
出版信息
Br J Vener Dis. 1982 Apr;58(2):109-12. doi: 10.1136/sti.58.2.109.
The combination of culture on modified New York City (MNYC) medium and identification of neisserial isolates by the Phadebact gonococcus test was evaluated in routine laboratory practice. The sensitivity of coagglutination was 96.7% (318/329 isolates) and that of immunofluorescence (IF) 97.9% (322/329 isolates); the specificity of both methods was 96.8% (120/124 isolates). Of the 329 gonococcal isolates, 286 (86.9%) could be tested by coagglutination and 309 (93.9%) by IF after only 24 hours' incubation. Identification by coagglutination from primary cultures on MNYC medium is considered to be very rapid, simple, and efficient for the cultural diagnosis of anogenital gonorrhoea in women and urethral gonorrhoea in men. Because of the high prevalence of meningococci in the pharynx and their not infrequent occurrence in the anorectum of homosexual men the identity of isolates from these sites is best confirmed by sugar utilisation tests.
在常规实验室实践中，对改良纽约市（MNYC）培养基上的培养与用Phadebact淋球菌试验鉴定奈瑟菌分离株的联合方法进行了评估。协同凝集的敏感性为96.7%（329株分离株中的318株），免疫荧光（IF）的敏感性为97.9%（329株分离株中的322株）；两种方法的特异性均为96.8%（124株分离株中的120株）。在MNYC培养基上进行原代培养后，仅培养24小时，329株淋球菌分离株中，286株（86.9%）可用协同凝集法检测，309株（93.9%）可用IF法检测。对于女性肛门生殖器淋病和男性尿道淋病的培养诊断，通过MNYC培养基上原代培养物的协同凝集进行鉴定被认为非常快速、简单且有效。由于咽部脑膜炎球菌的高流行率以及它们在同性恋男性肛门直肠中并不罕见的出现情况，来自这些部位的分离株的鉴定最好通过糖利用试验来确认。
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