Chang H H, Robinson A R, Common R H
Can J Biochem. 1975 Feb;53(2):223-30. doi: 10.1139/o75-031.
The phytoestrogen, diadzein, was injected intramuscularly as [4-14-C]daidzein into two laying hens. The radioactive materials in the urine for the succeeding 23 (hen 1) or 14 (hen 2) days were fractionated on a DEAE-Sephadex column by a gradient of NaCl and the fractions thus separated were further analyzed by solvent partition, susceptability to enzymic cleavage and thin-layer chromatography. The sic following components were identified and quantitated: [14-C]diadzein, [14-C]equol, [14-C]diadzein monosulfate, [14-C]equol monosulfate, [14-C]diazein disulfate, and [14-C]equol disulfate. The urine from hen 2 yielded also the sulfate of an unidentified conversion product of [14-C]daidzein. Repeared tests for glucuronides of [14-C]daidzein or its conversion products gave negative results, excluding the possibility that any appreciable proportion of the radioactivity in the urine was in the form of beta-glucuronide. It is concluded that the diadzein and the equol excretion in the urine of the laying hen are present for the most part as monosulfates and disulfates.
将植物雌激素黄豆苷元以[4-¹⁴C]黄豆苷元的形式肌肉注射到两只产蛋母鸡体内。在接下来的23天（母鸡1）或14天（母鸡2）内，收集尿液中的放射性物质，通过NaCl梯度在DEAE-葡聚糖凝胶柱上进行分级分离，然后对分离得到的级分进一步通过溶剂分配、酶解敏感性和薄层色谱法进行分析。鉴定并定量了以下六种成分：[¹⁴C]黄豆苷元、[¹⁴C]雌马酚、[¹⁴C]黄豆苷元单硫酸盐、[¹⁴C]雌马酚单硫酸盐、[¹⁴C]黄豆苷元二硫酸盐和[¹⁴C]雌马酚二硫酸盐。母鸡2的尿液中还产生了[¹⁴C]黄豆苷元一种未鉴定转化产物的硫酸盐。对[¹⁴C]黄豆苷元或其转化产物的葡萄糖醛酸苷进行的重复测试结果为阴性，排除了尿液中任何可观比例的放射性以β-葡萄糖醛酸苷形式存在的可能性。得出的结论是，产蛋母鸡尿液中的黄豆苷元和雌马酚排泄产物大部分以单硫酸盐和二硫酸盐的形式存在。
