Chang H H, Robinson A R, Chan A H, Common R H
Can J Biochem. 1977 Jan;55(1):50-5. doi: 10.1139/o77-009.
The phytoestrogen formononetin was injected intramuscularly as [4-14C]formononetin into two adult hens. Radioactive materials in the urine for the succeeding 14 days (hen 1) or 16 days (hen 2) were fractionated on DEAE-Sephadex-25 columns by elution with a gradient of NaCl; the four major fractions thus separated were examined by solvent partition, thin-layer chromatography, and enzymic cleavage. The following seven radioactive components were identified in the urine, the average proportions of each being given in terms of percentage of total 14C recovered from the urine: [14C]formononetin (4.3%); [14C]diaidzein (11.4%); [14C]equol (6.8%); [14C]daidzein monosulfate (30.4%); [14C]equol monosulfate (5.8%); [14C]diadzein disulfate (19.8%); and [14C]equol disulfate (6.5%). Small proportions of sulfates of unidentified radioactive phenols were present. Tests for presence of glucosiduronates of 14C-labelled material gave negative results. Radioactive formononetin sulfate was not detected in the urine of either hen.
将植物雌激素刺芒柄花素以[4-¹⁴C]刺芒柄花素的形式肌肉注射到两只成年母鸡体内。在接下来的14天（母鸡1）或16天（母鸡2）内，通过用NaCl梯度洗脱，在DEAE-葡聚糖-25柱上对尿液中的放射性物质进行分级分离；通过溶剂分配、薄层色谱和酶解对如此分离出的四个主要级分进行检测。在尿液中鉴定出以下七种放射性成分，每种成分的平均比例以从尿液中回收的总¹⁴C的百分比表示：[¹⁴C]刺芒柄花素（4.3%）；[¹⁴C]二氢大豆黄酮（11.4%）；[¹⁴C]雌马酚（6.8%）；[¹⁴C]大豆黄酮单硫酸盐（30.4%）；[¹⁴C]雌马酚单硫酸盐（5.8%）；[¹⁴C]二氢大豆黄酮二硫酸盐（19.8%）；以及[¹⁴C]雌马酚二硫酸盐（6.5%）。存在少量未鉴定放射性酚类的硫酸盐。对¹⁴C标记物质的葡萄糖醛酸苷的检测结果为阴性。在两只母鸡的尿液中均未检测到放射性刺芒柄花素硫酸盐。
