Carvalho L M, Linhares A X, Trigo J R
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, CEP 13083-970 Campinas, SP, Brazil.
Forensic Sci Int. 2001 Aug 15;120(1-2):140-4. doi: 10.1016/s0379-0738(01)00421-2.
Larvae of Chrysomya albiceps (Wiedemann) and Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae) were reared on tissues from rabbits administered twice the lethal dosage of diazepam in order to study the effects of this drug on the development of these two species. The rabbits were given 50mg of diazepam via ear vein infusion. From 18 to 54h, larvae feeding on tissues containing the drug developed more rapidly than larvae from the control colony for both fly species. The time required for pupariation and adult emergence was significantly greater for colony fed on tissues from diazepam dosed rabbits than for the control ones. These differences are significant for they are large enough to alter the estimate of postmortem interval based on fly development. The presence of diazepam could be detected through gas chromatography-mass spectrometer (GC-MS) in all rabbit samples and in almost all diptera samples in this experiment.
为了研究地西泮对白头金蝇（Chrysomya albiceps (Wiedemann)）和恶臭金蝇（Chrysomya putoria (Wiedemann)）（双翅目：丽蝇科）这两个物种发育的影响，在给兔子注射两倍致死剂量的地西泮后，用兔子的组织饲养这两种金蝇的幼虫。通过耳静脉输注给兔子注射50毫克地西泮。在18至54小时内，取食含药组织的两种金蝇幼虫比对照群体的幼虫发育得更快。取食注射地西泮的兔子组织的群体化蛹和羽化所需时间比对照组显著更长。这些差异很显著，因为它们大到足以改变基于蝇类发育的死后间隔估计。在本实验中，通过气相色谱 - 质谱联用仪（GC - MS）可以在所有兔子样本以及几乎所有双翅目样本中检测到地西泮的存在。
