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自动化红细胞抗体检测。一种改进方法。

Automated red cell antibody detection. An improved method.

作者信息

Dawes B J, Goldsmith K L

出版信息

Vox Sang. 1975;29(4):248-52. doi: 10.1111/j.1423-0410.1975.tb00505.x.

DOI:10.1111/j.1423-0410.1975.tb00505.x
PMID:1146281
Abstract

A single-channel AutoAnalyzer was used to screen potential blood-grouping sera to exclude the presence of unwanted antibodies. In order to detect the widest possible range of antibodies, a low-ionic strength Polybrene method was employed, but difficulty was encountered because the aggregated cells adhered to the glass surfaces of the reaction coils, resulting in instability of the system. Modifications were made to the apparatus, and the improvements in the demonstration of antibodies that resulted are described.

摘要

使用单通道自动分析仪筛选潜在的血型血清,以排除不需要的抗体。为了检测尽可能广泛的抗体范围,采用了低离子强度聚凝胺方法,但遇到了困难,因为聚集的细胞附着在反应管的玻璃表面,导致系统不稳定。对该仪器进行了改进,并描述了由此带来的抗体检测方面的改进。

相似文献

1
Automated red cell antibody detection. An improved method.自动化红细胞抗体检测。一种改进方法。
Vox Sang. 1975;29(4):248-52. doi: 10.1111/j.1423-0410.1975.tb00505.x.
2
Polybrene technique for red cell antibody screening using microplates.使用微孔板进行红细胞抗体筛查的聚凝胺技术。
J Clin Pathol. 1988 May;41(5):556-7. doi: 10.1136/jcp.41.5.556.
3
[Automated screening and identification of irregular anti-erythrocyte antibodies].
Rev Fr Transfus Immunohematol. 1982 Jun;25(3):297-308. doi: 10.1016/s0338-4535(82)80015-9.
4
[Detection of irregular alloantibodies by Groupamatic using a trypsin-polybrene-citrate technic].
Rev Fr Transfus Immunohematol. 1978 Mar;21(2):457-72. doi: 10.1016/s0338-4535(78)80084-1.
5
Comparison of a modified manual hexadimethrine bromide (Polybrene) and a low-ionic-strength solution antibody detection technique.
Transfusion. 1985 Mar-Apr;25(2):145-8. doi: 10.1046/j.1537-2995.1985.25285169208.x.
6
The manual polybrene test: a simple and rapid procedure for detection of red cell antibodies.
Transfusion. 1980 Mar-Apr;20(2):206-11. doi: 10.1046/j.1537-2995.1980.20280169962.x.
7
Association between HL-A and red cell antigens. An autoanalyzer study.HL-A与红细胞抗原之间的关联。一项自动分析仪研究。
Vox Sang. 1974 Feb;26(2):97-106. doi: 10.1111/j.1423-0410.1974.tb02674.x.
8
Antiglobulin test in low-ionic strength salt solution for rapid antibody screening and cross-matching.低离子强度盐溶液中的抗球蛋白试验用于快速抗体筛查和交叉配血。
Vox Sang. 1974;26(1):53-61. doi: 10.1111/j.1423-0410.1974.tb02666.x.
9
Limitations of Polybrene to detect ABO incompatibility.
Vox Sang. 1986;51(4):318-20. doi: 10.1111/j.1423-0410.1986.tb01976.x.
10
Red blood cell antibody screening with groupamatic system. II. A two-step haemagglutination technique using a trypsin-polybren-citrate method.
Vox Sang. 1981 Jan;40(1):34-43. doi: 10.1111/j.1423-0410.1981.tb00666.x.

引用本文的文献

1
Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system.在低离子强度-硫酸鱼精蛋白自动分析仪系统中,通过使用范围扩展和更长的滤光波长改进抗体检测。
J Clin Pathol. 1976 Nov;29(11):991-4. doi: 10.1136/jcp.29.11.991.