一种短暂的组蛋白高乙酰化信号在体内标记核小体，以便在PHO8启动子处进行重塑。

A transient histone hyperacetylation signal marks nucleosomes for remodeling at the PHO8 promoter in vivo.

作者信息

Reinke H, Gregory P D, Hörz W

机构信息

Institut für Physiologische Chemie, Universität München, Germany.

出版信息

Mol Cell. 2001 Mar;7(3):529-38. doi: 10.1016/s1097-2765(01)00200-3.

DOI:10.1016/s1097-2765(01)00200-3

PMID:11463378

Abstract

Chromatin remodeling of the yeast PHO8 promoter requires the SAGA histone acetyltransferase complex. We report here that SAGA is necessary and sufficient to establish an activator-dependent hyperacetylation peak over the PHO8 promoter that is restricted to those nucleosomes that are remodeled upon activation. This local hyperacetylated state is observed upon activation in the absence of the SWI/SNF complex when the remodeling process is frozen subsequent to activator binding. Hyperacetylation is lost, however, if remodeling is permitted to go to completion. Thus, a transient histone hyperacetylation signal is shown to be a prerequisite for, and determinant of, the domain of nucleosome remodeling in vivo.

摘要

酵母PHO8启动子的染色质重塑需要SAGA组蛋白乙酰转移酶复合体。我们在此报告，SAGA对于在PHO8启动子上建立一个依赖激活因子的高乙酰化峰是必要且充分的，该高乙酰化峰仅限于那些在激活时发生重塑的核小体。当激活因子结合后重塑过程被冻结时，在没有SWI/SNF复合体的情况下激活时可观察到这种局部高乙酰化状态。然而，如果允许重塑完成，高乙酰化就会消失。因此，瞬时组蛋白高乙酰化信号被证明是体内核小体重塑区域的先决条件和决定因素。