Krebs J E, Fry C J, Samuels M L, Peterson C L
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605, USA.
Cell. 2000 Sep 1;102(5):587-98. doi: 10.1016/s0092-8674(00)00081-7.
Regulation of eukaryotic gene expression requires ATP-dependent chromatin remodeling enzymes, such as SWI/SNF, and histone acetyltransferases, such as Gcn5p. Here we show that SWI/SNF remodeling controls recruitment of Gcn5p HAT activity to many genes in late mitosis and that these chromatin remodeling enzymes play a role in regulating mitotic exit. In contrast, interphase expression of GAL1, HIS3, PHO5, and PHO8 is accompanied by SWI/SNF-independent recruitment of Gcn5p HAT activity. Surprisingly, prearresting cells in late mitosis imposes a requirement for SWI/SNF in recruiting Gcn5p HAT activity to the GAL1 promoter, and GAL1 expression also becomes dependent on both chromatin remodeling enzymes. We propose that SWI/SNF and Gcn5p are globally required for mitotic gene expression due to the condensed state of mitotic chromatin.
真核基因表达的调控需要ATP依赖的染色质重塑酶，如SWI/SNF，以及组蛋白乙酰转移酶，如Gcn5p。我们在此表明，SWI/SNF重塑在有丝分裂后期控制Gcn5p HAT活性向许多基因的募集，并且这些染色质重塑酶在调控有丝分裂退出中发挥作用。相比之下，GAL1、HIS3、PHO5和PHO8在间期的表达伴随着Gcn5p HAT活性的不依赖SWI/SNF的募集。令人惊讶的是，在有丝分裂后期使细胞预阻滞会导致在将Gcn5p HAT活性募集到GAL1启动子方面对SWI/SNF产生需求，并且GAL1的表达也变得依赖于这两种染色质重塑酶。我们提出，由于有丝分裂染色质的凝聚状态，SWI/SNF和Gcn5p对于有丝分裂基因表达是全局必需的。
