ccn3（nov）原癌基因在人前列腺细胞系和组织中的差异表达。
Differential expression of the ccn3 (nov) proto-oncogene in human prostate cell lines and tissues.
作者信息
Maillard M, Cadot B, Ball R Y, Sethia K, Edwards D R, Perbal B, Tatoud R
机构信息
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
出版信息
Mol Pathol. 2001 Aug;54(4):275-80. doi: 10.1136/mp.54.4.275.
AIMS
To investigate the expression of the human ccn3 (hccn3; nov) proto-oncogene, a member of the CCN family of proteins, in prostate epithelial cells and prostate tissue samples.
METHODS
Normal adult prostate luminal epithelial cells immortalised by SV40 large T (PNT1A and PNT1B), metastatic tumours (LNCaP, DU-145, and PC-3), and prostate tissue samples from patients with benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma were used. hccn3 (nov) mRNA was measured by the reverse transcription polymerase chain reaction (RT-PCR) and hCCN3 (NOV) protein expression was determined by immunochemistry.
RESULTS
hccn3 (nov) RNA values were higher in PC-3 cells than in the other prostate cell lines. The immortalised normal cell lines either did not express hccn3 (nov) RNA (PNT1B) or expressed very low amounts (PNT1A). BPH samples expressed variable amounts of hccn3 (nov) RNA. With the use of immunocytochemistry, all cell lines except PNT1A and PNT1B were shown to contain hCCN3 (NOV) protein. hCCN3 (NOV) was localised mainly in the epithelial compartment of BPH and adenocarcinoma samples, and there was evidence of luminal secretion.
CONCLUSION
The overexpression of hccn3 (nov) RNA in cancer cell lines compared with other cell lines and its epithelial localisation in human prostate samples are consistent with a role for hCCN3 (NOV) in prostatic tumorigenesis.
目的
研究人CCN3（hCCN3；nov）原癌基因（CCN蛋白家族成员之一）在前列腺上皮细胞和前列腺组织样本中的表达情况。
方法
使用经SV40大T抗原永生化的正常成年前列腺腔上皮细胞（PNT1A和PNT1B）、转移性肿瘤细胞（LNCaP、DU - 145和PC - 3）以及来自良性前列腺增生（BPH）和前列腺腺癌患者的前列腺组织样本。通过逆转录聚合酶链反应（RT - PCR）检测hccn3（nov）mRNA，通过免疫化学法测定hCCN3（NOV）蛋白表达。
结果
PC - 3细胞中的hccn3（nov）RNA值高于其他前列腺细胞系。永生化的正常细胞系要么不表达hccn3（nov）RNA（PNT1B），要么表达量极低（PNT1A）。BPH样本中hccn3（nov）RNA的表达量各不相同。通过免疫细胞化学检测发现，除PNT1A和PNT1B外，所有细胞系均含有hCCN3（NOV）蛋白。hCCN3（NOV）主要定位于BPH和腺癌样本的上皮区域，并有腔内分泌的证据。
结论
与其他细胞系相比，hccn3（nov）RNA在癌细胞系中的过表达及其在人前列腺样本中的上皮定位，与hCCN3（NOV）在前列腺肿瘤发生中的作用一致。
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