Shih C C, Shih C M, Chen Y L, Su Y Y, Shih J S, Kwok C F, Lin S J
Institute of Clinical Medicine, National Yang-Ming University School of Medicine, Taipei 112, Taiwan.
J Biomed Mater Res. 2001 Nov;57(2):200-7. doi: 10.1002/1097-4636(200111)57:2<200::aid-jbm1159>3.0.co;2-5.
The potential cytotoxicity on vascular smooth muscle cells of corrosion products from 316 L stainless steel, one of most popular biomaterials of intravascular stents, has not been highlighted. In this investigation, 316 L stainless steel wires were corroded in Dulbecco's modified eagle's medium with applied constant electrochemical breakdown voltage, and the supernatant and precipitates of corrosion products were prepared as culture media. The effects of different concentrations of corrosion products on the growth of rat aortic smooth muscle cells were conducted with the [3H]-thymidine uptake test and cell cycle sorter. Both the supernatant and precipitates of corrosion products were toxic to the primary culture of smooth muscle cells. The growth inhibition was correlated well with the increased nickel ions in the corrosion products when nickel concentration was above 11.7 ppm. The corrosion products also changed cell morphology and induced cell necrosis. The cell growth inhibition occurred at the G0/G1 to S transition phase. Similar to our recent study of nitinol stent wire, the present investigation also demonstrated the cytotoxicity of corrosion products of 316 L stainless steel stent wire on smooth muscle cells, which might affect the poststenting vascular response.
作为血管内支架最常用的生物材料之一，316L不锈钢腐蚀产物对血管平滑肌细胞的潜在细胞毒性尚未得到充分关注。在本研究中，将316L不锈钢丝在添加了恒定电化学击穿电压的杜氏改良 Eagle 培养基中进行腐蚀，并将腐蚀产物的上清液和沉淀物制备成培养基。通过[3H] - 胸腺嘧啶核苷摄取试验和细胞周期分选仪研究了不同浓度的腐蚀产物对大鼠主动脉平滑肌细胞生长的影响。腐蚀产物的上清液和沉淀物对平滑肌细胞的原代培养均具有毒性。当镍浓度高于11.7 ppm时，生长抑制与腐蚀产物中镍离子含量的增加密切相关。腐蚀产物还改变了细胞形态并诱导细胞坏死。细胞生长抑制发生在G0/G1期至S期的转变阶段。与我们最近对镍钛诺支架丝的研究相似，本研究也证明了316L不锈钢支架丝的腐蚀产物对平滑肌细胞具有细胞毒性，这可能会影响支架置入后的血管反应。
