Bánfalvi Z, Kostyál Z, Barta E
Agricultural Biotechnology Center, Hungary.
Mol Gen Genet. 1994 Nov 15;245(4):517-22. doi: 10.1007/BF00302265.
The patatin gene is the best known "tuber-specific" gene of potato (Solanum tuberosum). Patatin is encoded by a multigene family that can be divided into two classes. Class I genes are highly expressed in tubers and are sucrose inducible, while class II genes are under developmental control and are expressed mainly in root tips. Here we report the isolation and characterization of cDNA clones corresponding to a patatin gene of the non-tuberizing Solanum species S. brevidens. We show that the gene is 94-100% homologous to the class I type patatin genes of S. tuberosum; the homology includes the sequences in the 5' and the 3' untranslated regions. However, the patatin gene of S. brevidens is regulated like class II type patatin genes and cannot be transcriptionally activated by elevated levels of sucrose. This result further supports the idea that the components required for tuberization may be present in non-tuberizing solanaceous plants, but are regulated differently.
马铃薯块茎贮藏蛋白基因是马铃薯（Solanum tuberosum）中最著名的“块茎特异性”基因。马铃薯块茎贮藏蛋白由一个多基因家族编码，该家族可分为两类。I类基因在块茎中高度表达且受蔗糖诱导，而II类基因受发育调控，主要在根尖表达。本文报道了与非块茎形成的茄属物种短柄茄（Solanum brevidens）的一个马铃薯块茎贮藏蛋白基因相对应的cDNA克隆的分离与鉴定。我们发现该基因与马铃薯的I类马铃薯块茎贮藏蛋白基因具有94%-100%的同源性；这种同源性包括5'和3'非翻译区的序列。然而，短柄茄的马铃薯块茎贮藏蛋白基因的调控方式与II类马铃薯块茎贮藏蛋白基因相似，不能被蔗糖水平升高转录激活。这一结果进一步支持了这样一种观点，即块茎形成所需的成分可能存在于非块茎形成的茄科植物中，但调控方式不同。
