Bánfalvi Z, Molnár A, Kostyál Z, Lakatos L, Molnár G
Agricultural Biotechnology Center, Gödölló, Hungary.
Acta Biol Hung. 1997;48(1):77-86.
A method for synchronized in vitro tuber induction in a Hungarian cultivar of Solanum tuberosum designated "Keszthelyi 855" has been developed. It was shown that in this system tuberization and stolon elongation primarily depend on the level of sucrose in the medium. The cytokinin, 6-bensylaminopurine (BAP), also enhances the efficiency of tuber formation, however, only at sucrose concentration above 4% (w/v). The synchronized plant culture provided starting material for isolation of genes specifically expressed in tuberizing Solanum species during the early stage of tuber development. In comparison with the non-tuberizing Solanum brevidens, three types of specific transcripts have been obtained by differential screening. Based on DNA sequence analysis the genes isolated code for the major tuber proteins, patatin and proteinase inhibitors.
已开发出一种用于匈牙利马铃薯品种“Solanum tuberosum Keszthelyi 855”同步体外块茎诱导的方法。结果表明，在该系统中，块茎形成和匍匐茎伸长主要取决于培养基中蔗糖的水平。细胞分裂素6-苄基氨基嘌呤（BAP）也能提高块茎形成的效率，然而，仅在蔗糖浓度高于4%（w/v）时才有效。同步化的植物培养物为分离在块茎发育早期在块茎形成的茄属物种中特异性表达的基因提供了起始材料。与不形成块茎的Solanum brevidens相比，通过差异筛选获得了三种类型的特异性转录本。基于DNA序列分析，分离出的基因编码主要的块茎蛋白、马铃薯球蛋白和蛋白酶抑制剂。
