粘连形成与内皮型一氧化氮合酶的表达
Adhesion development and the expression of endothelial nitric oxide synthase.
作者信息
Svinarich D M, Zaher F M, Holmdahl L, Chegini N, Gonik B, Diamond M P
机构信息
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
出版信息
Infect Dis Obstet Gynecol. 2001;9(2):113-6. doi: 10.1155/S1064744901000199.
OBJECTIVE
This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.
METHODS
Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and beta-actin mRNA levels.
RESULTS
eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were < or = 3.12 x 10(-4), < or = 3.12 x 10(-4), 6.24 x 10(-4) and 2.5 x 10(-3) attomoles/microl, respectively. Beta-actin mRNA levels for all tissues were between 1.25 x 10(-1) and 6.25 x 10(-2) attomoles/microl.
CONCLUSION
eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.
目的
本研究旨在确定一氧化氮（NO），一种强力血管舒张剂和血栓形成抑制剂，是否参与粘连的形成与维持。
方法
从手术患者身上采集皮肤、皮下组织、腹膜及粘连组织，提取总RNA。采用定量逆转录聚合酶链反应（QRT-PCR）对内皮型一氧化氮合酶（eNOS）和β-肌动蛋白mRNA水平进行定量分析。
结果
皮肤、皮下组织、腹膜及粘连组织的eNOS mRNA水平分别为≤3.12×10⁻⁴、≤3.12×10⁻⁴、6.24×10⁻⁴和2.5×10⁻³阿托摩尔/微升。所有组织的β-肌动蛋白mRNA水平在1.25×10⁻¹至6.25×10⁻²阿托摩尔/微升之间。
结论
在组织粘连中可检测到eNOS mRNA，因此其可能在粘连的形成与维持中发挥作用。
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