海胆沙钱克隆组蛋白DNA的部分变性图谱分析
Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliaris.
作者信息
Portmann R, Schaffner W, Birnstiel M
出版信息
Nature. 1976 Nov 4;264(5581):31-4. doi: 10.1038/264031a0.
DOI:10.1038/264031a0
PMID:1069909
Abstract
A cloned 5.6-kb DNA repeat unit of Psammechinus miliaris containing all five histone coding sequences of known order and polarity has been shown to fall clearly into a low melting and a high melting half by thermal denaturation. The topoligies of the DNA sequences thus defined were determined by partial denaturation mapping in the electron microscope to gain further insight into the distribution of spacers and genes within histone DNA.
摘要
一种克隆的海胆5.6千碱基对DNA重复单元，包含所有已知顺序和极性的五个组蛋白编码序列，通过热变性已被证明明显分为低熔点和高熔点两部分。通过电子显微镜中的部分变性图谱确定由此定义的DNA序列的拓扑结构，以进一步深入了解组蛋白DNA中间隔区和基因的分布。
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