铜绿假单胞菌外切核酸酶IX：一种在乙二胺四乙酸存在下具有活性的独特脱氧核糖核酸酶。
PaeExo IX: a unique deoxyribonuclease from Pseudomonas aeruginosa active in the presence of EDTA.
作者信息
Scurlock T R, Miller R V
出版信息
Nucleic Acids Res. 1979 Sep 11;7(1):167-77. doi: 10.1093/nar/7.1.167.
Abstract
A new deoxyribonuclease, PaeExo IX, has been purified to electrophoretic homogeneity from extracts of Pseudomonas aeruginosa strain PAO. This enzyme, which is active in the presence of EDTA, is equally efficient in hydrolyzing native and heart-denatured DNA to acid-s-luble products. The enzyme is partially or totally inhibited by the presence of several divalent cations. The active protein has a molecular weight of 1.6 +/- 0.1 x 10(5) and is composed of two nonidentical polypeptides with molecular weights of 78,000 and 69,000.
摘要
一种新的脱氧核糖核酸酶PaeExo IX已从铜绿假单胞菌PAO菌株的提取物中纯化至电泳纯。这种酶在EDTA存在下具有活性，在将天然DNA和热变性DNA水解为酸溶性产物方面同样有效。几种二价阳离子的存在会部分或完全抑制该酶的活性。活性蛋白的分子量为1.6 +/- 0.1 x 10(5)，由两条分子量分别为78,000和69,000的不同多肽组成。
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