Qiu Y H, Peng Y P, Cheng C, Dai L
Department of Physiology, Nantong Medical College, Nantong 226001.
Sheng Li Xue Bao. 1999 Aug;51(4):477-80.
In the present study, restraint, which caused no direct tissue damage, was selected as a method of stress to investigate time effect of stress-induced suppression of lymphocyte transformation in rats. The lymph node and spleen extract or the serum from rats restrained for 3, 6, 12, 18 h or 12, 24, 48, 72, 96 h after restraint, with concanavalin A (Con A), was added to the lymphocyte suspension of rat mesenteric lymph node and was incubated for 72 h. The optical density (OD) value indicating the level of lymphocyte transformation was determined by colorimetric assay of methylthiazol tetrazolium bromide (MTT). The results are as follows. (1) The lymph node and spleen extract or the serum from the rats restrained for 3 or 6 h did not markedly affect the lymphocyte transformation. (2) The lymph node and spleen extract or the serum from the rats restrained for 12 h or 18 h significantly inhibited the lymphocyte transformation and stronger inhibition occurred at 18 h. (3) Up to 96 h after restraint, the suppressive effect of the lymph node or spleen extract on the lymphocyte transformation still existed, but the suppression of the serum on the lymphocyte transformation almost disappeared. These results suggest that the restraint of rats may cause lymphoid tissues to produce some immune suppressive protein of stress, which is released into blood leading to a generalized suppression of cellular immunity.
在本研究中，选择不会造成直接组织损伤的束缚作为应激方法，以研究应激诱导大鼠淋巴细胞转化抑制的时间效应。将束缚3、6、12、18小时或束缚后12、24、48、72、96小时的大鼠的淋巴结和脾脏提取物或血清，与伴刀豆球蛋白A（Con A）一起加入大鼠肠系膜淋巴结的淋巴细胞悬液中，并孵育72小时。通过溴化甲基噻唑四氮唑（MTT）比色法测定表示淋巴细胞转化水平的光密度（OD）值。结果如下。（1）束缚3或6小时的大鼠的淋巴结和脾脏提取物或血清对淋巴细胞转化没有明显影响。（2）束缚12小时或18小时的大鼠的淋巴结和脾脏提取物或血清显著抑制淋巴细胞转化，且在18小时时抑制作用更强。（3）束缚后长达96小时，淋巴结或脾脏提取物对淋巴细胞转化的抑制作用仍然存在，但血清对淋巴细胞转化的抑制作用几乎消失。这些结果表明，束缚大鼠可能导致淋巴组织产生一些应激免疫抑制蛋白，这些蛋白释放到血液中导致细胞免疫的全身性抑制。
