Zhang Jie, Wang Jian, Feng Juan, Zhong Zhen, Zhang Bo, Fan Shao-Guang
Department of Physiology, The Health Science Center of Peking University, Beijing 100083.
Sheng Li Xue Bao. 2002 Apr 25;54(2):171-4.
Our previous work demonstrated that under the conditions of restraint stress and under the control of central nervous system (CNS), an immune suppressive protein of stress (ISPS) was generated in peripheral lymph tissue and released into the blood stream, acting as an immune suppressor. In the present work, a protein similar to ISPS was found in human tonsil (a peripheral lymph tissue). Human tonsil was homogenized and the extract was prepared. It was found that lymphocyte proliferation was significantly suppressed by the extract. The suppression induced by the extract was partially reversed by the monoclonal antibody against ISPS (2C4). In ELISA test, the extract was able to bind to the monoclonal antibody. By immunohistochemistry, many ISPS positive cells were found in human tonsil. The ISPS positive cells were also found in human lymph nodes. Taken together, all the results demonstrate that a protein similar to ISPS may exist in human peripheral lymphoid tissue.
我们之前的研究表明，在束缚应激条件下且受中枢神经系统（CNS）调控时，外周淋巴组织中会产生一种应激免疫抑制蛋白（ISPS），并释放到血流中，作为一种免疫抑制剂发挥作用。在本研究中，在人扁桃体（一种外周淋巴组织）中发现了一种与ISPS相似的蛋白质。将人扁桃体匀浆并制备提取物。发现该提取物能显著抑制淋巴细胞增殖。提取物诱导的抑制作用被抗ISPS单克隆抗体（2C4）部分逆转。在酶联免疫吸附测定（ELISA）试验中，提取物能够与单克隆抗体结合。通过免疫组织化学方法，在人扁桃体中发现了许多ISPS阳性细胞。在人淋巴结中也发现了ISPS阳性细胞。综上所述，所有结果表明人外周淋巴组织中可能存在一种与ISPS相似的蛋白质。
