Zha H B, Xu H, Zhai Q Z, Chen W, Fan S G
Department of Physiology, Beijing Medical University.
Sheng Li Xue Bao. 1991 Feb;43(1):31-7.
In order to study the effect of stress on lymphocyte proliferation, SD rats were restrained with four limbs tied on a frame in supine position at room temperature (20 degrees C) for 20 h, and control animals were not disturbed in home cage. The blood was then collected from the heart under light ether anesthesia. The peripheral blood lymphocytes were separated from heparinized whole blood by density gradient (d 1.077) centrifugation, or the serum was obtained after the blood coagulated at 4 degrees C for about 6h. It was found that the blood lymphocyte proliferation induced by Con A was significantly inhibited in the stressed group as compared with the control (P less than 0.01, n = 8, ANOVA). The result was in accordance with our earlier study in which the animals were stressed with electric shock. In the present study, it was also found that the serum of the stressed animals was capable of suppressing Con A-induced lymphocyte proliferation of normal mice (P less than 0.01, n = 8, ANOVA) to a significant extent. Thus the present experiment suggests that there is some substance with suppressive activity on lymphocyte proliferation in the serum of the stressed rats. The serum lost its suppressive activity when it was heated to 100 degrees C (3 min), treated with 60% methanol or incubated with trypsin (64 micrograms/ml), thus suggesting that the suppressive factor(s) most likely is a kind of protein.
为研究应激对淋巴细胞增殖的影响，将SD大鼠四肢绑在框架上，于室温（20℃）仰卧位束缚20小时，对照动物在饲养笼中不做处理。然后在轻度乙醚麻醉下从心脏采血。通过密度梯度（d 1.077）离心从肝素化全血中分离外周血淋巴细胞，或将血液在4℃凝固约6小时后获得血清。结果发现，与对照组相比，应激组中Con A诱导的血液淋巴细胞增殖受到显著抑制（P<0.01，n = 8，方差分析）。该结果与我们早期用电休克使动物产生应激的研究一致。在本研究中，还发现应激动物的血清能够显著抑制正常小鼠Con A诱导的淋巴细胞增殖（P<0.01，n = 8，方差分析）。因此，本实验表明应激大鼠血清中存在某种对淋巴细胞增殖具有抑制活性的物质。当血清加热至100℃（3分钟）、用60%甲醇处理或与胰蛋白酶（64微克/毫升）孵育时，其抑制活性丧失，这表明抑制因子很可能是一种蛋白质。
