Bennink J R, Doherty P C
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3482-5. doi: 10.1073/pnas.76.7.3482.
After depletion of alloreactive potential, immunologically naive T cells from C57BL/6J (Kb-Db) mice (B6) can be induced to respond to vaccinia virus in the context of both H-2Kk and H-2Db when stimulated in B10.A(4R) (Kk-Db) recipients. However, negatively selected B10.A(2R) (Kk-Db) T cells respond to H-2Db-vaccinia virus but not to H-2Kb-vaccinia virus when primed in an irradiated B6 environment. The B6 mouse strain is a high responder to vaccinia virus associated with H-2Db, whereas the B10.A(2R) and B10.A(4R) recombinants are low responders. Responsiveness in the context of H-2Db is thus recognized when the only homology between T cell and recipient is at the H-2D locus and is suppressed when H-2Kk is also present in both situations. The fact that negatively selected H-2Kb-Db T cells can be induced to recognize H-2Kk-vaccinia virus may reflect the existence of an "altered self" complex which is recognized via a single receptor, perhaps drawn from an alloreactive T-cell repertoire. At least in some instances, patterns of T-cell responsiveness are not totally constrained by the spectrum of H-2 antigens encountered in thymus.
在清除同种异体反应潜能后,当在B10.A(4R)(Kk-Db)受体中受到刺激时,来自C57BL/6J(Kb-Db)小鼠(B6)的免疫未成熟T细胞在H-2Kk和H-2Db的背景下均可被诱导对痘苗病毒产生反应。然而,经阴性选择的B10.A(2R)(Kk-Db)T细胞在受照射的B6环境中致敏时,对H-2Db-痘苗病毒有反应,但对H-2Kb-痘苗病毒无反应。B6小鼠品系是与H-2Db相关的痘苗病毒的高反应者,而B10.A(2R)和B10.A(4R)重组体是低反应者。因此,当T细胞与受体之间的唯一同源性位于H-2D位点时,在H-2Db背景下的反应性可被识别,而当两种情况下都存在H-2Kk时,反应性则受到抑制。经阴性选择的H-2Kb-Db T细胞可被诱导识别H-2Kk-痘苗病毒这一事实可能反映了一种“改变的自身”复合物的存在,该复合物通过单一受体被识别,该受体可能来自同种异体反应性T细胞库。至少在某些情况下,T细胞反应模式并不完全受胸腺中遇到的H-2抗原谱的限制。