鲁比达atum对小鼠的免疫抑制作用。（注：这里“rubidatum”可能是一个特定药物或物质名称，原词拼写是否有误存疑，正常翻译为“鲁比达atum”，若这不是你想要的结果，请检查原文并告知。）

Immunosuppressive effects of rubidatum in mice.

作者信息

Yang S L, Liu F, Zhang H, Dou Q L

机构信息

Department of Internal Medicine, 474th Hospital, PLA, Urumqi 830011, China.

出版信息

Acta Pharmacol Sin. 2000 Aug;21(8):765-8.

PMID:11501189

Abstract

AIM

To study the effect of rubidatum (Rub) on immune function in normal mice.

METHODS

Serum lysozyme concentration (SLC) was measured using micrococcus lysodiekticus as a substrate. Delayed type hypersensitivity (DTH) was determined by measuring the thickness of the right hind footpad 24 h after the injection of 1 x 10(8) washed sRBC (50 microL 10% sRBC). Serum hemolysin concentration was determined by OD measuring at A540 after the serum was treated with 2-mercaptoethanol. Phagocytic function of peripheral leukocyte (Leu) were determined by the incorporated radioactivity of [3H]TdR. The hemolytic activity of plaque forming cell (PFC) was determined by measuring the lymphocytemediated hemolysis of sheep red blood cell in vitro. T- and B-lymphocyte transformation (TLT and BLT) were induced by phytohemagglutinin (PHA) and lipopolysacharide (LPS) respectively and measured by the incorporation of [3H]TdR.

RESULTS

Rub 125, 500, 2000 mg.kg-1.d-1 p.o. to BALB/c (or NIH) mice decreased the SLC; inhibited the phagocytosing functions of peripheral leukocytes; diminished the hemolytic activity of PFC; decreased the HC50; inhibited the DTH reaction; and showed inhibitory effects on TLT and BLT.

CONCLUSION

Rub has immunosuppressive effects on immune system in mice by affecting M phi, T, and B lymphocyte, which suggests that Rub has inhibitory effects on both nonspecific and specific immune function.

摘要

目的

研究玉红膏（Rub）对正常小鼠免疫功能的影响。

方法

以溶壁微球菌为底物测定血清溶菌酶浓度（SLC）。通过在注射1×10⁸个洗涤过的sRBC（50 μL 10% sRBC）24小时后测量右后足垫厚度来测定迟发型超敏反应（DTH）。用2-巯基乙醇处理血清后，通过在A540处测量OD来测定血清溶血素浓度。通过[³H]TdR的掺入放射性来测定外周白细胞（Leu）的吞噬功能。通过测量体外绵羊红细胞的淋巴细胞介导的溶血来测定空斑形成细胞（PFC）的溶血活性。分别用植物血凝素（PHA）和脂多糖（LPS）诱导T淋巴细胞和B淋巴细胞转化（TLT和BLT），并通过[³H]TdR的掺入来测量。