青蒿琥酯对小鼠免疫功能的影响。
Effects of artesunate on immune function in mice.
作者信息
Lin P Y, Feng Z M, Pan J Q, Zhang D, Xiao L Y
机构信息
Department of Pharmacology, Guangzhou Institute of Medicine and Health, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 Sep;16(5):441-4.
AIM
To study the effects of artesunate (dihydroartemisinine-12-alpha-succinate, Art) on immune function in mice.
METHODS
Hemolysin concentration was determined by colorimetric method. Serum IgG and C3 contents were measured by single immunodiffusion method. Percentage of lymphocyte transformation, phagocytosis percentage and phagocytic index were counted under microscope.
RESULTS
Art im 75 mg kg-1 bid x 7 d decreased the humolysin-forming capacity and levels of serum IgG of mice sensitized with sheep red blood cell. The serum complement 3 level rose remarkably, when Art was given im to Plasmodium berghei-infected mice. Art enhanced the PHA-induced lymphocyte transformation rate (in vivo) in mice and increased the weight of spleen but reduced that of thymus in mice. Art elevated the DNFB-induced delayed-type hypersensitivity. Art im 75 mg kg-1 bid x 5 d reduced the percentage of phagocytosis of peritoneal macrophages and the phagocytic index.
CONCLUSION
Art suppressed the humoral immune responses but enhanced the cell-mediated immunity.
目的
研究青蒿琥酯（二氢青蒿素 - 12 - α - 琥珀酸酯，青蒿琥酯）对小鼠免疫功能的影响。
方法
采用比色法测定溶血素浓度。采用单向免疫扩散法测定血清IgG和C3含量。在显微镜下计数淋巴细胞转化率、吞噬百分率和吞噬指数。
结果
腹腔注射青蒿琥酯75mg/kg，每日2次，连续7天，可降低用绵羊红细胞致敏的小鼠的溶血素形成能力和血清IgG水平。给感染伯氏疟原虫的小鼠腹腔注射青蒿琥酯时，血清补体3水平显著升高。青蒿琥酯可提高小鼠PHA诱导的淋巴细胞转化率（体内），增加小鼠脾脏重量，但降低胸腺重量。青蒿琥酯可提高DNFB诱导的迟发型超敏反应。腹腔注射青蒿琥酯75mg/kg，每日2次，连续5天，可降低腹腔巨噬细胞的吞噬百分率和吞噬指数。
结论
青蒿琥酯抑制体液免疫反应，但增强细胞免疫。
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