内核酸酶活性的变异性表明，反刍月形单胞菌自然种群内具有高度的遗传多样性。

Variability of endonucleolytic activity indicates high genetic diversity within the natural population ofSelenomonas ruminantium.

机构信息

Institute of Animal Physiology, Slovak Academy of Sciences, 040 01, Kosice, Slovakia.

出版信息

Folia Microbiol (Praha). 1997 Apr;42(2):121-4. doi: 10.1007/BF02898719.

Abstract

The population of bacteria ofSelenomonas ruminantium species in the rumen of fallow-deer was analyzed using endonucleolytic activity assay and plasmid profiles. This analysis indicated a high diversity within the population ofS. ruminantium. At least 12 different restriction profiles, indicating the presence of the different specificity nucleases, have been observed. Site-specific endonucleases were detected in 17 out of 45 strains tested. In other strains a various level of nonspecific activity was detected. Plasmid DNAs ranging in size from 0.9 to more than 25 kbp were detected in 60% of strains analyzed. No or little correlation was observed between the endonuclease activity and the plasmid content. The presence of different specificity endonucleases, as well as differences of plasmid profiles of isolates possessing identical specific activity indicate that the population ofS. ruminantium in the rumen of an individual animal consists of at least 10 different clones.

摘要

采用内切核酸酶活性分析和质粒图谱分析的方法，研究了空齿鹿瘤胃中反刍月形单胞菌种群的细菌数量。该分析表明，反刍月形单胞菌种群内具有高度多样性。至少观察到 12 种不同的限制图谱，表明存在不同特异性核酸酶。在所测试的 45 株菌株中，检测到 17 株具有特异性内切核酸酶。在其他菌株中，检测到不同程度的非特异性活性。在所分析的 60%的菌株中，检测到大小从 0.9kbp 到 25kbp 以上的质粒 DNA。未观察到内切核酸酶活性与质粒含量之间存在相关性或相关性很小。不同特异性内切核酸酶的存在以及具有相同特异性活性的分离株的质粒图谱的差异表明，单个动物瘤胃中的反刍月形单胞菌种群至少由 10 个不同的克隆组成。