Restriction endonucleases from Selenomonas ruminantium which recognize and cleave 5'-AT/TAAT-3'.
作者信息
Pristas P, Vanat I, Godany A, Javorský P
机构信息
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
出版信息
Arch Microbiol. 1994;161(5):439-41. doi: 10.1007/BF00288956.
PMID:8042908
Abstract
Two natural isolates from fallow-deer rumen identified as Selenomonas ruminantium were found to produce a restriction endonuclease which we called Sru4DI. This enzyme was isolated from cell extracts by phosphocellulose chromatography. Analysis of the Sru4DI recognition site showed that Sru4DI recognizes the hexanucleotide sequence 5'-AT/TAAT-3' generating 5'dinucleotide protruding ends upon cleavage and thus is a true isoschizomer of vspI, a restriction enzyme isolated from Vibrio sp.
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