Tola S, Crobeddu S, Chessa G, Uzzau S, Idini G, Ibba B, Rocca S
Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", Sassari, Italy.
FEMS Microbiol Lett. 2001 Aug 7;202(1):45-50. doi: 10.1111/j.1574-6968.2001.tb10778.x.
Mycoplasma agalactiae, the causative agent of contagious agalactia in small ruminants, produces a protein, named P80, that is detectable in all wild-type isolates examined to date and that appears expressed during the early phase of infection. We describe here the identification, cloning and expression of the gene encoding P80 (ma-mp81). The deduced amino acid sequence is consistent with a hydrophobic and basic protein that possesses a lipoprotein signal peptide. Sequence analysis of gene ma-mp81 suggests that P80 is a membrane lipoprotein that shows significant homology with other putative lipoproteins of M. pneumoniae. An internal 1-kb fragment of ma-mp81 was expressed in Escherichia coli as a 6xHis-tagged protein. The purified recombinant protein greatly reacted with polyclonal anti-P80 sera raised in lamb.
无乳支原体是小反刍兽疫的病原体，它产生一种名为P80的蛋白质，在迄今检测的所有野生型分离株中均可检测到，并且在感染早期似乎就已表达。我们在此描述编码P80的基因（ma-mp81）的鉴定、克隆和表达。推导的氨基酸序列与具有脂蛋白信号肽的疏水碱性蛋白一致。基因ma-mp81的序列分析表明，P80是一种膜脂蛋白，与肺炎支原体的其他假定脂蛋白具有显著同源性。ma-mp81的一个1 kb内部片段在大肠杆菌中表达为带有6xHis标签的蛋白质。纯化的重组蛋白与在羔羊中产生的多克隆抗P80血清发生强烈反应。
