Joseph J A, Appel J B
Pharmacol Biochem Behav. 1976 Jul;5(1):35-7. doi: 10.1016/0091-3057(76)90285-9.
Forty naive male albino rats were trained to press a bar on a fixed-ratio (FR 32) schedule of water reinforcement. They were then divided into two groups, one of which (N = 20) received 5 min of extra water 12 hr before each experimental session; the other group (N = 20) received no extra water. Half of the animals in each group was given three daily doses (100 mg/kg) of the trytophan hydroxylase inhibitor p-chlorophenylalanine methyl ester (PCPA) while the remaining animals were given control injections of the PCPA vehicle. Ten days following the last administration of PCPA (or vehicle) all animals were given a low dose of LSD (20 mug/kg). Bar-pressing behavior was significantly disrupted only in those animals receiving both PCPA and extra water. Central (whold brain) concentrations of serotonin (5-HT) were significantly lower in all animals which had been treated with PCPA. These results, along with those previously reported, suggest that amount of deprivation can be an important determinant of both the ability of drugs to alter behavior and the dependence of such alterations upon underlying neuronal activity.
四十只未经训练的雄性白化大鼠被训练在固定比率（FR 32）的水强化程序下按压杠杆。然后将它们分成两组，其中一组（N = 20）在每次实验前12小时额外给予5分钟的水；另一组（N = 20）不给予额外的水。每组一半的动物每天接受三次剂量（100毫克/千克）的色氨酸羟化酶抑制剂对氯苯丙氨酸甲酯（PCPA），而其余动物则注射PCPA溶媒作为对照。在最后一次给予PCPA（或溶媒）十天后，所有动物都给予低剂量的麦角酸二乙酰胺（LSD，20微克/千克）。只有那些同时接受PCPA和额外水的动物，其按压杠杆行为才受到显著干扰。所有接受PCPA治疗的动物，其大脑（全脑）血清素（5 - HT）浓度均显著降低。这些结果，连同先前报道的结果表明，剥夺量可能是药物改变行为能力以及这种改变对潜在神经元活动依赖性的重要决定因素。
