Fernández-Presas A M, Zavala J T, Fauser I B, Merchant M T, Guerrero L R, Willms K
Departamento de Microbiologia and Parasitologia, Facultad de Medicina, Universidad Nacional Autónoma de México, Cuidad Universitaria, México.
Parasitol Res. 2001 Aug;87(8):619-25. doi: 10.1007/s004360100409.
The susceptibility of Trypanosoma cruzi epimastigotes to lysis by normal or immune sera in a complement-dependent reaction has been reported, but the effects induced directly by immune serum depleted of complement remain unstudied. The aim of this work was to study the ultrastructural alterations induced in T. cruzi epimastigotes by immune mouse or rabbit sera with or without complement. A local isolate of T. cruzi (Queretaro) was used in all experiments. Immune sera were raised in both mouse and rabbit by immunization with T. cruzi epimastigote antigens. Light microscopy showed intense agglutination of epimastigotes when incubated with decomplemented mouse or rabbit immune sera. A distinctive ultrastructural feature of this agglutination pattern was the fusion of plasma membranes and a pattern of intercrossing between subpellicular microtubules. Agglutination was associated with fragmentation of nuclear membranes and swelling of cytoplasm, Golgi cisternae, endoplasmic reticulum, mitochondria and kinetoplast membranes. Agglutinated parasites also incorporated trypan blue stain. Results of [3H]-thymidine incorporation confirmed that epimastigotes exposed to specific antibodies in the absence of complement were incapable of proliferating. Ultrastructural changes observed in epimastigote micrographs incubated with decomplemented immune mouse sera were statistically significant (P<0.001) when compared with results obtained from images after incubation with decomplemented normal mouse sera.
已有报道称克氏锥虫前鞭毛体在补体依赖反应中对正常血清或免疫血清的裂解敏感，但补体缺失的免疫血清直接诱导的效应尚未得到研究。本研究的目的是探究有或无补体的免疫小鼠或兔血清对克氏锥虫前鞭毛体诱导的超微结构改变。所有实验均使用了克氏锥虫（克雷塔罗株）的本地分离株。通过用克氏锥虫前鞭毛体抗原免疫小鼠和兔来制备免疫血清。光学显微镜观察显示，当与补体缺失的小鼠或兔免疫血清孵育时，前鞭毛体出现强烈凝集。这种凝集模式的一个独特超微结构特征是质膜融合以及表膜下微管的交叉模式。凝集与核膜破碎以及细胞质、高尔基体池、内质网、线粒体和动质体膜肿胀有关。凝集的寄生虫也摄取了台盼蓝染料。[3H] - 胸腺嘧啶核苷掺入实验结果证实，在无补体情况下暴露于特异性抗体的前鞭毛体无法增殖。与补体缺失的正常小鼠血清孵育后的图像结果相比，补体缺失的免疫小鼠血清孵育的前鞭毛体显微照片中观察到的超微结构变化具有统计学意义（P<0.001）。
