一种新型标记技术揭示了组蛋白H2A/H2B二聚体尾部结构域在体内染色质组装中的功能。
A novel labeling technique reveals a function for histone H2A/H2B dimer tail domains in chromatin assembly in vivo.
作者信息
Thiriet C, Hayes J J
机构信息
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, USA.
出版信息
Genes Dev. 2001 Aug 15;15(16):2048-53. doi: 10.1101/gad.910201.
Abstract
During S phase in eukaryotes, assembly of chromatin on daughter strands is thought to be coupled to DNA replication. However, conflicting evidence exists concerning the role of the highly conserved core histone tail domains in this process. Here we present a novel in vivo labeling technique that was used to examine the role of the amino-terminal tails of the H2A/H2B dimer in replication-coupled assembly in live cells. Our results show that these domains are dispensable for nuclear import but at least one tail is required for replication-dependent, active assembly of H2A/H2B dimers into chromatin in vivo.
摘要
在真核生物的S期，子链上染色质的组装被认为与DNA复制相关联。然而，关于高度保守的核心组蛋白尾部结构域在此过程中的作用，存在相互矛盾的证据。在此，我们展示了一种新型的体内标记技术，该技术用于研究H2A/H2B二聚体的氨基末端尾部在活细胞中复制偶联组装过程中的作用。我们的结果表明，这些结构域对于核输入并非必需，但在体内，至少一条尾部是H2A/H2B二聚体依赖复制的、活跃组装到染色质中所必需的。
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本文引用的文献
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Nuclear import of histone H2A and H2B is mediated by a network of karyopherins.组蛋白H2A和H2B的核输入由核转运蛋白网络介导。
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