Yin E T
J Lab Clin Med. 1975 Sep;86(3):430-4.
The Limulus amebocyte lysate, a proteinaceous composite isolated from the hemolymph cells of the horseshoe crab (Limulus polyphemus) is sensitive to picogram quantities of Gram-negative bacterial lipopolysaccharides. However, a controversy currently exists as to whether the Limulus amebocyte lysate is specifically sensitive to Gram-negative bacterial endotoxins as a result of a recent report that the blood coagulation protease, thrombin, can mimic endotoxins in the Limulus amebocyte lysate test. Experiments including those employing two highly purified fractions isolated from the Limulus lystae have provided us with evidence that thrombin per se is unable to mimic endotoxin.
鲎试剂是一种从鲎（美洲鲎）的血淋巴细胞中分离出来的蛋白质复合物，对皮克量的革兰氏阴性菌脂多糖敏感。然而，目前存在一个争议，即鲎试剂是否对革兰氏阴性菌内毒素具有特异性敏感性，因为最近有报道称，凝血蛋白酶凝血酶在鲎试剂试验中可以模拟内毒素。包括那些使用从鲎试剂中分离出的两种高度纯化组分的实验，为我们提供了证据，证明凝血酶本身无法模拟内毒素。
