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通过化学方法提高电沉淀(电凝)的响应性。

Increased responsiveness of electric precipitation (electrosynerese) by means of a chemical method.

作者信息

Ottó S, Verzár R, Somogyi E

出版信息

Z Rechtsmed. 1979 Jul 17;83(2):101-4. doi: 10.1007/BF02092265.

DOI:10.1007/BF02092265
PMID:115172
Abstract

Electric precipitation is a method in serological diagnostics (determination of species-specificity). Since serum protein fractions (especially immunoglobulins) and extracts from blood stains migrate in agar-gel at pH 8.2--8.6 towards the cathode they cannot be used for the determination of the species specificity. By a chemical method using glutaraldehyde authors have produced an albumin-immunoglobulin complex, altering in this way the direction of electrophoretic mobility. Treatment of the blood samples with glutaraldehyde increases the sensitivity and realiability of the method.

摘要

电沉淀法是血清学诊断(物种特异性测定)中的一种方法。由于血清蛋白组分(尤其是免疫球蛋白)和血渍提取物在pH 8.2 - 8.6的琼脂凝胶中向阴极迁移,因此它们不能用于物种特异性的测定。作者通过使用戊二醛的化学方法制备了白蛋白 - 免疫球蛋白复合物,从而改变了电泳迁移的方向。用戊二醛处理血样可提高该方法的灵敏度和可靠性。

相似文献

1
Increased responsiveness of electric precipitation (electrosynerese) by means of a chemical method.通过化学方法提高电沉淀(电凝)的响应性。
Z Rechtsmed. 1979 Jul 17;83(2):101-4. doi: 10.1007/BF02092265.
2
Determination of immunoglobulin subclasses by electrosyneresis.
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3
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本文引用的文献

1
PRECIPITIN REACTIONS IN FORENSIC PROBLEMS. A NEW METHOD FOR PRECIPITIN REACTIONS ON FORENSIC BLOOD, SEMEN AND SALIVA STAINS.法医问题中的沉淀素反应。一种针对法医血迹、精液斑和唾液斑进行沉淀素反应的新方法。
Nature. 1964 Mar 14;201:1092-3. doi: 10.1038/2011092a0.
2
[Description of a technic simultaneously combining electrophoresis and immunological precipitation in gel: electrosyneresis].[一种在凝胶中同时结合电泳和免疫沉淀的技术描述:电凝缩]
Biochim Biophys Acta. 1959 Jul;34:258-60. doi: 10.1016/0006-3002(59)90261-6.
3
The medico-legal importance of immunological cross reactions.
免疫交叉反应的法医学重要性。
Z Rechtsmed. 1974 Jun 18;74(3):161-5. doi: 10.1007/BF00200584.
4
Electroimmunoquantitation of serum proteins cross-linked by glutaraldehyde.
J Immunol Methods. 1975 Nov;9(1):69-79. doi: 10.1016/0022-1759(75)90037-x.